Abstract
MICRO-INJECTION of certain strategic substances into the axon of the squid (Loligo pealii) has been carried out in order to study further the correlations between observed electrical activity of the nerve and possible neuro-chemical events. A micro-pipette with a long shaftlet having a lumen at the tip not more than 5 µ in diameter can be readily inserted into the giant axon between a pair of external electrodes. At will, it can be placed at the site of one electrode. These manœuvres can be accomplished without damage to excitation of, or propagation in, the fibre. The present communication is confined to reporting the blocking action of eserine and prostigmine, stilbamidine and curare, injected in aqueous solutions (usually containing 0.2 per cent phenol red, which colours the injection site an orange-yellow). Total fluid injected varied from about 10−4 mm.3 to 10 or 12 mm.3, usually, however, being of the order of 0.5–5 mm.3.
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References
Bullock, T. H., Nachmansohn, D., and Rothenberg, M. A., J. Neurophysiol., 9, 9 (1946).
Bergmann, F., Wilson, I. B., and Nachmansohn, D., Biochim. et Biophys. Acta, 6, 217 (1950).
Grundfest, H., First Conference on the Nerve Impulse, 39 (Josiah Macy, Jr., Foundation, N.Y., 1951).
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GRUNDFEST, H., NACHMANSOHN, D., KAO, C. et al. Mode of Blocking of Axonal Activity by Curare and Inhibitors of Acetyl-cholinesterase. Nature 169, 190 (1952). https://doi.org/10.1038/169190a0
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DOI: https://doi.org/10.1038/169190a0
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