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Combined Phase-contrast and Interference-contrast Microscopy

Abstract

IN interference microscopy1, contrast in transparent objects is obtained by adding a coherent wave of a certain phase and amplitude to the wave which has passed through the object. In the usual vector representation2, this is equivalent to shifting the origin of the vector circle representing the image. It is clear that, in principle at least, it is possible to add further vectors representing other coherent waves and thus to shift the origin to different new positions. We may thus have consecutive or successive interference contrast.

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References

  1. Merton, T., Proc. Roy. Soc., A, 189, 309 (1947); A, 191, 1 (1947). Dyson, J., Proc. Roy. Soc., A, 204, 170 (1950). Philpot, J. St. L., “Progress in Biophysics”, 1, 142 (1950).

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  2. Barer, R., Proc. Internat. Colloquium on Phase Contrast and Interference Contrast (Paris, 1951): Optita Acta (in the press).

  3. Payne, B. O., J. Roy. Micr. Soc., 70, 255 (1950). Oettlé, A. G., J. Roy. Micr. Soc., 70, 232 (1950).

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BARER, R. Combined Phase-contrast and Interference-contrast Microscopy. Nature 169, 108–109 (1952). https://doi.org/10.1038/169108a0

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