Abstract
IT has been shown that the natural phenolic œstrogens produce fluorescence on being heated with phosphoric acid1,2. When applying this method to urinary extracts, it was found that a non-specific fluorescence obscures the readings for the œstrogens, especially in the ‘strong phenolic fraction’—the œstriol fraction. On attempting to purify the œstrogenic fractions of the non-specific fluorescence, we observed that a great part of it was non-phenolic, but was due to a ‘neutral’ substance or substances, which were carried over in the partition procedure then used (a modified Bachmann–Pettit method2) to the œstrogenic fraction.
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References
Finkelstein, M., Hestrin, S., and Koch, W., Proc. Soc. Exp. Biol. and Med., 64, 64 (1947).
Finkelstein, M., Proc. Soc. Exp. Biol. and Med., 69, 181 (1948).
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ZONDEK, B., FINKELSTEIN, M. Determination and Occurrence of Fluorescent Substances in the Neutral Fraction of Urinary Extracts. Nature 168, 831–832 (1951). https://doi.org/10.1038/168831a0
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DOI: https://doi.org/10.1038/168831a0
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