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Transfer of Bacterial Antibodies from the Hen to the Chick

Abstract

DURING an investigation on the transfer of antibodies against Salmonella pullorum from the hen to egg yolk, non-agglutinating antibodies as well as agglutinins were found in the hen's serum and in the yolk. The non-agglutinating antibodies were detected in serum by the method of Morgan and Schutze1. The serum was diluted serially, and an equal volume of antigen added to each dilution; after 18 hr. at 53° C. any agglutination was noted, and the antigen in those tubes in which there was no agglutination was washed; toeach of those tubes was added anti-avian-globulin serum. If the antigen had been sensitized by the unknown serum, it was agglutinated in the presence of the anti-avian-globulin serum. Antibodies of this type are referred to as non-agglutinating antibodies.

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References

  1. Morgan, W. T. J., and Schutze, H., Brit. J. Exp. Path., 27, 286 (1946).

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  2. Needham, J., “Biochemistry and Morphogenesis” (Univ. Press, Cambridge, 1942).

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  3. Marza, V. D., and Marza, E. V., Quart. J. Micro. Sci., 78, 133 (1935).

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  4. Rosenheim, A. H., Biochem. J., 31, 54 (1937).

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BUXTON, A. Transfer of Bacterial Antibodies from the Hen to the Chick. Nature 168, 657–658 (1951). https://doi.org/10.1038/168657a0

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