Abstract
THE lipase method of Gomori1 has been little used in morbid histology. This communication is concerned with the results of a preliminary study of the distribution of lipase in human tissue using Gomori's method. The method, which is one of his ingenious techniques using the deposition of an insoluble calcium salt at the site of enzyme activity, is based on the splitting of a water-soluble ester of higher fatty acids and conversion of the calcium soap to lead sulphide. The original method1 and the modification of Wachstein2 were satisfactory, using as substrate Tween 40 or 60 as recommended by them, or Crill 6 or 7, which is a similar British product (Croda, Ltd., Goole, Yorks).
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References
Gomori, G., Proc. Soc. Exp. Biol. N.Y., 58, 362 (1945).
Wachstein, J. Exp. Med., 84, 25 (1946).
Mark, Arch. Path., 49, 545 (1950).
Gomori, G., Arch. Path., 41, 121 (1946).
Danielli, J. F., Nature, 165, 762 (1950).
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SNEATH, P. Histochemical Demonstration of Lipase. Nature 166, 699–700 (1950). https://doi.org/10.1038/166699a0
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DOI: https://doi.org/10.1038/166699a0
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