Abstract
THE isolation of the ketonic fraction of biological materials by the use of trimethylcarbohydrazidomethylammonium chloride (reagent ‘T’) has been described by Girard and Sandulesco1. Essentially, the method utilizes the formation of water-soluble, ether-insoluble hydrazones between the ketonic bodies and the reagent, from which the non-ketonic fraction is removed by repeated ether extraction. This complex compound is then decomposed by treatment with mineral acid, and the resulting ketonic fraction extracted with ether.
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References
Girard and Sandulesco, Helv. Chem. Acta, 19, 1095 (1936).
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WALTERS, C. Removal of Girad Reagent ‘T’ from Biological Ketonic Fractions not easily Extracted by Organic Solvents. Nature 166, 604–605 (1950). https://doi.org/10.1038/166604a0
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DOI: https://doi.org/10.1038/166604a0
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