Abstract
IN attempting to culture excised immature plant embryos, it is frequently desirable to avoid autoclaving the nutrilite components (for example, malt extract). These are usually Seitz-filtered separately and transferred by pipette to the individual culture vials containing the previously autoclaved sugar and inorganic components of the medium. To workers unskilled in bacteriological methods, this represents a tedious and time-consuming operation.
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STEPHENS, S. A Simple Apparatus for Adding Seitz-Filtered Components Aseptically to Embryo Culture Media. Nature 162, 932 (1948). https://doi.org/10.1038/162932b0
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DOI: https://doi.org/10.1038/162932b0
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