Abstract
PREVIOUS work on the ultra-violet microscopy of living cells has led to conflicting conclusions as to the value of this technique in cytological research. Thus, Wyckoff, Ebeling and Ter Louw1 reported that Feulgen staining, which demonstrates desoxyribose-nucleic acid in fixed cells, duplicates the results obtained by the ultra-violet microscopy of living cells. "Ultra-violet microscopy can be considered as the equivalent of a vital staining specific for formed chromatin" was their conclusion. More recently, in Nature, Brumberg and Larionow2 maintained that desoxyribose-nucleic acid is present in the nucleus of living cells in a form which does not absorb irradiation in the 2600 A. region, and that absorption only occurs in injured and dead cells.
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References
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Brumberg, E. M., and Larionow, L. T., Nature, 158, 663 (1947).
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Barnard, J. E., and Welch, F. V., "Practical Photomicrography" (London: Edward Arnold, 1936).
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LUDFORD, R., SMILES, J. & WELCH, F. Ultra-Violet Microscopy of Living Malignant Cells. Nature 162, 650–651 (1948). https://doi.org/10.1038/162650a0
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DOI: https://doi.org/10.1038/162650a0
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