New Colour and Fluorescence Reactions in the Steroid and Synthetic Å’strogen Series

Abstract

BY using suitable solvents as diluents in the reaction mixture to modify the phosphoric test for natural strogens¹, new and apparently specific colour or fluorescence tests have been found for trans transδ ^{5 : 6}-androstenediol-3 : 17, dienœstrol, 17-ethinyl œstradiol, 17-methyl androstenediol-3 : 17, d-l-equilin, striol, 16-keto œstradiol, œstrololactone acetate, and Westerfeld‘s lactone acetate. The use of low temperatures in two of these reactions may be advantageous in the examination of biological extracts. Conditions have been found for the quantitative estimation of androstenediol and dienœstrol. Further Work on the quantitative estimation of the other compounds is in progress and will be published elsewhere.