Loss of White Cells in Bone Marrow Culture

Abstract

MANY techniques of cell culture involve suspension of cells in some fluid medium, and incubation in sterile glass containers, with subsequent counting of the suspended cells. Although it has long been known that certain cells are very ‘sticky' and can adhere most firmly to glass surfaces¹, this fact seems to have been neglected in work with cell cultures, where some cells might be lost by irreversible adhesion to the walls of the culture vessel. The following is an attempt to investigate this point in bone-marrow culture ; here adhesion is of particular importance because only certain types of haemopoietic cells are ‘sticky'.