Abstract
IT was pointed out previously that in the unstained living human spermatid the Golgi apparatus is sometimes discernible with bright-ground illumination, as an area slightly differing in refractive index from the remainder of the cytoplasm1. Recently, I have been able to confirm and extend these observations with the phase-contrast microscope. This instrument was of the phase-accelerating or positive type, so that dark and light regions in the image indicated higher and lower refractive indices, as a rule.
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References
Oettlé, A. G., S. Afr. J. Sci., 37, 239 (1940).
Gatenby, J. B., and Beams, H. W., Quart. J. Mic. Sci., 78, 1 (1936).
Baker, J. R., Quart. J. Mic. Sci., 85, 1 (1944).
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OETTLÉ, A. Golgi Apparatus of Living Human Testicular Cells Seen with Phase-Contrast Microscopy. Nature 162, 76–77 (1948). https://doi.org/10.1038/162076a0
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DOI: https://doi.org/10.1038/162076a0
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