Abstract
In recent years there have been many attempts to utilize the properties of the newer synthetic ion-exchange reagents for the separation of bases and amino-acids, but the application of truly chromato-graphic procedures has met with only partial success. In the course of the present investigation, it became clear that very sharp separations could be obtained on cation-exchange columns by the utilization of a strong base to displace others in the system. It was found that such a procedure could be reduced to the terms of the ‘displacement development’ principle introduced by Tiselius1 for separations on adsorption columns of carbon or alumina, and for this reason we have made use of the theoretical background developed by this worker and his colleagues.
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References
Tiselius, A., Ark. Kem. Min. Geol., 16 A, No. 18 (1943).
Claesson, S., Ark. Kem. Min. Geol., 23 A, No. 1 (1946).
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BENDALL, J., PARTRIDGE, S. & WESTALL, R. Displacement Chromatography on Cation-Exchange Materials. Nature 160, 374–375 (1947). https://doi.org/10.1038/160374a0
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DOI: https://doi.org/10.1038/160374a0
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