Abstract
IN the estimation of vitamin C in plant materials by titration with 2:6 dichlorophenolindophenol, it has hitherto been generally assumed that the dye titration value is sufficiently independent of the titration pH to make it unnecessary to control the latter at all precisely. The usual procedure of preparing extracts with metaphosphoric or trichloracetic acid solutions to inhibit the action of ascorbic acid oxidase results in a pH which may range from 0.8 to 1.5, or even higher, according to the experimental conditions.
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17 August 1946
An Erratum to this paper has been published: https://doi.org/10.1038/158233f0
References
Martius, C., and Euler, H. V., Biochem. Z., 271, 9 (1934).
Wokes, F., Organ, J. G., and Jacoby, F. C., J. Soc. Chem. Ind., 62, 232 (1943).
Mapson, L. W., J. Soc. Chem. Ind., 62, 223 (1943).
Melville, R., Wokes, F., and Organ, J. G., Nature, 152, 447 (1943).
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WOKES, F. Effect of pH in the Dye Titration of Vitamin C in Certain Plant Materials. Nature 158, 133 (1946). https://doi.org/10.1038/158133a0
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DOI: https://doi.org/10.1038/158133a0
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