Abstract
ELECTRON micrographs may be considered analogous to X-ray pictures, since the darkness and brightness depend on the thickness and density of the specimen; they are unlike micrographs taken with the light microscope, in which an image is formed due to differences in the amount of absorption or refraction within the object. The presence of very small particles in specimens for examination under the electron microscope will cause perceptible scattering, and the image formed of an object thicker than about 0·5 µ is merely an enlarged silhouette.
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DONOVAN, G. Biological Applications of the Electron Microscope*. Nature 154, 356–358 (1944). https://doi.org/10.1038/154356a0
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DOI: https://doi.org/10.1038/154356a0
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