Abstract
THE use of chlorazol black E as a stain for general purposes in ordinary microscopical technique was suggested by Prof. H. Graham Cannon in NATURE some years ago1. This nearly black substance agrees with many vital dyes in being a sulphonated acid azo-compound; but whereas trypan blue, trypan red, vital new red, diamine fast scarlet, benzo–purpurine 4B, the various Niagara blues, new Bordeaux L, Congo rubine, afridol blue, Baumwoll-rubin, etc., all possess two azo-linkages, chlorazol black E has three. It was thought interesting to try the trisazo compound as a vital dye, and the results suggest that it may be a useful one.
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References
Gannon, H. Graham, NATURE, 139, 549 (1937).
Gordon, H. K., and Chambers, R., J. Cell and Comp. Physiol., 17, 97 (1941).
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BAKER, J. Chlorazol Black E as a Vital Dye. Nature 147, 744 (1941). https://doi.org/10.1038/147744a0
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DOI: https://doi.org/10.1038/147744a0
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