Abstract
THE chemical determination of ascorbic acid is based upon the high reducing capacity shown by this substance with different organic compounds. One of the best known methods based upon this property is the titration of ascorbic acid with 2: 6 dichlorphenol indophenol1, after the removal of interfering substances with mercuric acetate. Others2 have found that ascorbic acid reacts with methylene blue, and this property was made use of for its estimation. This method appears to be the most satisfactory, because the presence of other reducing substances does not interfere with the reactions linked to certain hydrogen ion concentrations. It has, however, certain disadvantages inherent to its colorimetrical nature. It is on account of this circumstance that I have tried to modify this method of estimation and thus to eliminate possible sources of error.
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References
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M. van Eekelen and A. Emmerie, Biochem. J., 23, 268, 1153 (1934).
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GÁL, I. Estimation of Ascorbic Acid (Vitamin C) by Titration. Nature 138, 799 (1936). https://doi.org/10.1038/138799a0
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DOI: https://doi.org/10.1038/138799a0
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