Abstract
The activation of human 5-HT1C receptors by dopamine was studied in Xenopus oocytes voltage clamped at −70 mV. 5-HT1C receptors were expressed by microinjection of human cRNA transcripts (Biochem. Biophys. Res. Comm. 181:1469-1478, 1991) and allowing 2 to 4 days for expression. The application of 1 μM 5-HT or 1 mM dopamine did not cause any detectable current in 32 uninjected oocytes from 4 different frogs. On the other hand, the application of 5-HT or dopamine activated current responses in 20 cRNA injected oocytes from 3 different frogs. The activation of current by 5-HT or dopamine was concentration-dependent, having EC50 values of 6.2 nM and 67.7 μM, respectively. Although the EC50 value for dopamine was higher than 5-HT, the maximal current activated by dopamine was the same as the maximal current activated by 5-HT. Both 5-HT- (0.1 μM) and dopamine- (100 μM) activated currents were completely blocked by the 5-HT1C antagonist, 1-(1-Naphtyl)piperazine, (1 μM). The results suggest that dopamine directly activates human 5-HT1C receptors expressed in Xenopus oocytes.
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Oz, M., Zhang, L., Yu, D. et al. Dopamine Directly Activates Human 5-HT1C Receptors Expressed in Xenopus Oocytes. Neuropsychopharmacol 11, 279 (1994). https://doi.org/10.1038/sj.npp.1380190
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DOI: https://doi.org/10.1038/sj.npp.1380190