Abstract
I HAVE recently found that quinone (parabenzo-quinone) has an intense fixative action upon mitochondria. It is best to treat tissues for an hour with quinone dissolved in saline first, and then to transfer them to any fixative one may choose. I have found this much preferable to using quinone-saline alone or mixing it with any other fixative. The quinone may be at any concentration from 0·05 per cent for delicate tissues (such as kidney) to 0.5 per cent for liver. Plenty of fluid should be used. The saline should be roughly isotonic with the blood of the animal used, but if the quinone is used at 0.5 per cent, it is as well to make the saline 0.l per cent less concentrated than usual, so as to avoid having the fluid hypertonic. Surprisingly enough, Carnoy's fluid is one of the best fixatives to use after quinone. I have used the formula without chloroform.
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BAKER, J. A New Method for Mitochondria. Nature 130, 134 (1932). https://doi.org/10.1038/130134b0
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DOI: https://doi.org/10.1038/130134b0
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