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G-protein-coupled receptor-mediated activation of rap GTPases: characterization of a novel Gαi regulated pathway

Oncogene volume 23, pages 241–249 (2004)Cite this article

Abstract

Ras proteins mediate the proliferative effects of G-protein-coupled receptors (GPCRs), but the role of Rap proteins in GPCR signaling is unclear. We have developed a novel cellular proliferation assay for examining signal transduction to Rap utilizing Ras–rap chimeras that respond selectively to Rap-specific exchange factors, but which stimulate cellular proliferation through Ras effectors. Both the D1 dopamine receptor (Gs-coupled) and the 5HT1E serotonin receptor (Gi-coupled) mediated cellular proliferation in a Ras/rap chimera-dependent manner. Responses to both receptors were PKA-independent. Both receptors activated Ras/rap and full-length Rap as measured by activation-specific probes. Pertussis toxin blocked Ras/rap-dependent responses to 5HT1E but not D1. Ras/rap-dependent responses to both receptors were insensitive to β–γ scavengers. Responses to 5HT1E, but not D1, were sensitive to inhibition by a dominant-negative C3G fragment, by the Src-like kinase inhibitors PP1 and PP2, and by a dominant-negative mutant of Src. Very similar data were obtained for two other Gi-coupled receptors, the D2 dopamine receptor and the α2C adrenergic receptor. A constitutively active mutant of Gαi2 also mediated Ras/rap-dependent responses. These data indicate that GPCRs coupled to pertussis-toxin-sensitive G-proteins activate Rap through a Gα subunit, C3G, and Src-dependent pathway.

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Acknowledgements

We acknowledge J Bos, R Mattingly, N Nash, D Bar-Sagi, J Brugge, and P Stork for generous gifts of reagents.

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  1. ACADIA Pharmaceuticals Inc., 3911 Sorrento Valley Blvd, San Diego, 92121, CA, USA

    Jacques T Weissman, Jian-Nong Ma, Anthony Essex, Yan Gao & Ethan S Burstein

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  1. Jacques T Weissman
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  2. Jian-Nong Ma
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Correspondence to Ethan S Burstein.

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Weissman, J., Ma, JN., Essex, A. et al. G-protein-coupled receptor-mediated activation of rap GTPases: characterization of a novel Gαi regulated pathway. Oncogene 23, 241–249 (2004). https://doi.org/10.1038/sj.onc.1207014

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