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Reduced tumorigenicity of murine leukemia cells expressing protein-tyrosine phosphatase, PTPɛC

Oncogene volume 22, pages 1758–1762 (2003)Cite this article

Abstract

Recently, we reported that a cytosolic isoform of protein-tyrosine phosphatase ɛ (PTPɛC), when overexpressed, inhibits terminal differentiation and apoptosis of murine M1 myeloblastic leukemia cells induced by interleukin-6. To determine whether these observed effects in vitro correspond to a tumorigenicity of PTPɛC-expresser (M1-ɛC) cells in vivo, parent M1 and M1-ɛC cells were intravenously inoculated into scid or nude mice, and survival of mice receiving these cell lines was monitored. Unexpectedly, both scid and nude mice inoculated with M1-ɛC cells showed significantly prolonged survival time than those receiving parent M1 cells. While parent M1 cells inoculated by intravenous injection formed metastatic tumors in the spleen, expression of PTPɛC suppressed tumor development in the spleen. The results suggest a suppressive role of PTPɛC in tumorigenesis.

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References

  • Andersen JN, Elson A, Lammers R, Romer J, Clausen JT, Moller KB and Moller NP . (2001). Biochem. J., 354, 581–590.

  • Elson A . (1999). Oncogene, 18, 7535–7542.

  • Elson A and Leder P . (1995). Proc. Natl. Acad. Sci. USA, 92, 12235–12239.

  • Fischer EH, Charbonneau H and Tonks NK . (1991). Science, 253, 401–406.

  • Gil-Henn H, Volohonsky G and Elson A . (2001). J. Biol. Chem., 276, 31772–31779.

  • Gil-Henn H, Volohonsky G, Toledano-Katchalski H, Gandre S and Elson A . (2000). Oncogene, 19, 4375–4384.

  • Hoffman LB and Liebermann DA . (1991). Oncogene, 6, 903–909.

  • Hunter T and Cooper JA . (1985). Annu. Rev. Biochem., 54, 897–930.

  • Klingler-Hoffmann M, Fodero-Tavoletti MT, Mishima K, Narita Y, Cavenee WK, Furnari FB, Huang HJ and Tiganis T . (2001). J. Biol. Chem., 276, 46313–46318.

  • Krishnaraju K, Hoffman B and Liebermann DA . (1998). Blood, 92, 1957–1966.

  • LaMontagne Jr KR, Flint AJ, Franza Jr BR, Pandergast AM and Tonks NK . (1998a). Mol. Cell. Biol., 18, 2965–2975.

  • LaMontagne Jr KR, Hannon G and Tonks NK . (1998b). Proc. Natl. Acad. Sci. USA, 95, 14094–14099.

  • Lord KA, Abdollahi A, Hoffman-Liebermann B and Liebermann DA . (1993). Mol. Cell. Biol., 13, 841–851.

  • McArdle L, Rafferty M, Maelandsmo GM, Bergin O, Farr CJ, Dervan PA, O'Loughlin S, Herlyn M and Easty DJ . (2001). J. Invest. Dermatol., 117, 1255–1260.

  • Nakamura K, Mizuno Y and Kikuchi K . (1996). Biochem. Biophys. Res. Commun., 218, 726–732.

  • Neel BG and Tonks NK . (1997). Curr. Opin. Cell Biol., 9, 193–204.

  • Peretz A, Gil-Henn H, Sobko A, Shinder V, Attali B and Elson A . (2000). EMBO J., 19, 4036–4045.

  • Qiao S, Iwashita T, Furukawa T, Yamamoto M, Sobue G and Takahashi M . (2001). J. Biol. Chem., 276, 9460–9467.

  • Smith SI, Down M, Boyd AW and Li CL . (2000). Cancer Res., 60, 808–814.

  • Tanuma N, Nakamura K and Kikuchi K . (1999). Eur. J. Biochem., 259, 46–54.

  • Tanuma N, Nakamura K, Shima H and Kikuchi K . (2000). J. Biol. Chem., 275, 28216–28221.

  • Tanuma N, Shima H, Nakamura K and Kikuchi K . (2001). Blood, 98, 3030–3034.

  • Urushibara N, Karasaki H, Nakamura K, Mizuno Y, Ogawa K and Kikuchi K . (1998). Int. J. Oncol., 12, 603–607.

  • Warabi M, Nemoto T, Ohashi K, Kitagawa M and Hirokawa K . (2000). Exp. Mol. Pathol., 68, 187–195.

  • Zheng XM, Wang Y and Pallen CJ . (1992). Nature, 359, 336–339.

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Acknowledgements

We acknowledge Dr T Abo (Niigata University) for providing murine IFN-γ and Ajinomoto Co. (Yokohama, Japan) for IL-6. We are grateful to Dr M Hosokawa (Health Sciences University of Hokkaido) for critical reading of the manuscript. Thanks are also due to E Yoshida for secretarial assistance. This work was supported by a Grant-in-aid for Scientific Research on Priority Area (C) (2) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, Grant-in-aids for Scientific Research (B) and for Young Scientist (B) from the Japan Society for the Promotion of Science, and in part by a Grant from the Takeda Foundation.

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  1. Division of Biochemical Oncology and Immunology, Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-Ku, Sapporo, 060-0815, Japan

    Nobuhiro Tanuma, Hiroshi Shima & Kunimi Kikuchi

  2. Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-Ku, Sapporo, 060-0815, Japan

    Shigeki Shimada

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  1. Nobuhiro Tanuma
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Correspondence to Kunimi Kikuchi.

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Tanuma, N., Shima, H., Shimada, S. et al. Reduced tumorigenicity of murine leukemia cells expressing protein-tyrosine phosphatase, PTPɛC. Oncogene 22, 1758–1762 (2003). https://doi.org/10.1038/sj.onc.1206267

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