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Inhibition of ultraviolet B-mediated activation of nuclear factor κB in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-gallate

Oncogene volume 22, pages 1035–1044 (2003)Cite this article

Abstract

Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, possesses significant anti-inflammatory and cancer chemopreventive properties. Studies have shown the photochemopreventive effects of green tea and EGCG in cell culture, animal models, and human skin. The molecular mechanism(s) of photochemopreventive effects of EGCG are incompletely understood. We recently showed that EGCG treatment of the normal human epidermal keratinocytes (NHEK) inhibits ultraviolet (UV)B-mediated activation of the mitogen-activated protein kinase (MAPK) pathway. In this study, we evaluated the effect of EGCG on UVB-mediated modulation of the nuclear factor kappa B (NF-κB) pathway, which is known to play a critical role in a variety of physiological functions and is involved in inflammation and development of cancer. Immunoblot analysis demonstrated that the treatment of NHEK with EGCG (10–40 μ M) for 24 h resulted in a significant inhibition of UVB (40 mJ/cm2)-mediated degradation and phosphorylation of IκBα and activation of IKKα, in a dose-dependent manner. UVB-mediated degradation and phosphorylation of IκBα and activation of IKKα was also observed in a time-dependent protocol (15 and 30 min, 1, 2, 3, 6, 12 h post-UVB exposure). Employing immunoblot analysis, enzyme-linked immunosorbent assay, and gel shift assay, we demonstrate that EGCG treatment of the cells resulted in a significant dose- and time-dependent inhibition of UVB-mediated activation and nuclear translocation of a NF-κB/p65. Our data suggest that EGCG protects against the adverse effects of UV radiation via modulations in NF-κB pathway, and provide a molecular basis for the photochemopreventive effect of EGCG.

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  1. Farrukh Afaq, Vaqar M Adhami, Nihal Ahmad and Hasan Mukhtar: Part of this work was conducted while all authors were at the Department of Dermatology, Case Western Reserve University

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  1. Department of Dermatology, University of Wisconsin, Madison, 53706, WI, USA

    Farrukh Afaq, Vaqar M Adhami, Nihal Ahmad & Hasan Mukhtar

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  1. Farrukh Afaq
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Correspondence to Hasan Mukhtar.

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Afaq, F., Adhami, V., Ahmad, N. et al. Inhibition of ultraviolet B-mediated activation of nuclear factor κB in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-gallate. Oncogene 22, 1035–1044 (2003). https://doi.org/10.1038/sj.onc.1206206

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