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  • Oncogenomics
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An LOH and mutational investigation of the ST7 gene locus in human esophageal carcinoma

Oncogene volume 22, pages 467–470 (2003)Cite this article

Abstract

Frequent loss of heterozygosity (LOH) on human chromosome 7q31 has been reported in numerous malignancies. Suppressor of tumorigenicity 7 (ST7) has been identified as a candidate tumor suppressor gene in this region. To identify whether 7q31 and genetic alterations of ST7 were involved in human esophageal carcinogenesis, we performed LOH mapping of a 5.4 cM region at 7q31-q35 in 43 primary esophageal carcinomas, as well as mutational analyses of the ST7 gene in tumors with LOH in this region. Of 43 tumors, 12 (28%) showed LOH at 7q31–q35. These included four (22%) of 18 squamous cell carcinomas and eight (32%) of 25 adenocarcinomas. The peak LOH locus was D7S480, lying 4.2 Mb telomeric to ST7 and showing LOH in eight of 37 informative tumors, or 22%. No mutations were found in the entire coding or flanking intronic regions of the ST7 gene among 12 tumors with 7q-LOH. In addition, quantitative RT–PCR analyses of ST7 mRNA expression levels in 11/13 normal-tumor pairs failed to show more than a 50% decrease in tumor ST7 mRNA relative to matched normal tissues. These data suggest that LOH at 7q31–q35 is involved in the origin or progression of at least a subset of esophageal carcinomas, but that ST7 is not the target gene of this somatic event.

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Acknowledgements

This work was supported by USPHS grants CA95323, CA85069, CA77057, DK (to SJM), and the Medical Research Office, Department of Veterans Affairs (to SJM).

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Authors and Affiliations

  1. Department of Medicine, Division of Gastroenterology and Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD, USA

    Suna Wang, Yuriko Mori, Fumiaki Sato, Jing Yin, Yan Xu, Tong-Tong Zou, Andreea Olaru, Martha C Kimos, Florin M Selaru, Elena Deacu, John M Abraham, Bruce D Greenwald & Stephen J Meltzer

  2. Baltimore VA Hospital, Baltimore, 21201, Maryland, MD, USA

    Kellie Perry, Florin M Selaru, David Shibata, Bruce D Greenwald & Stephen J Meltzer

  3. Department of Surgery, Division of Surgical Oncology, Greenbaum Cancer Center, University of Maryland School of Medicine, Baltimore VA Hospital, Baltimore, 21201, MD, USA

    Kellie Perry & David Shibata

  4. Department of Surgery, Fudan University Medical College, Shanghai, China

    Ying-Chang Shi

  5. Department of Pathology, Fudan University Medical College, Shanghai, China

    Menghong Sun

Authors
  1. Suna Wang
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  2. Yuriko Mori
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  3. Fumiaki Sato
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  6. Tong-Tong Zou
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  7. Andreea Olaru
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  8. Martha C Kimos
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  9. Kellie Perry
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  10. Florin M Selaru
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  11. Elena Deacu
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  12. Menghong Sun
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  13. Ying-Chang Shi
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  14. David Shibata
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  15. John M Abraham
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  16. Bruce D Greenwald
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Correspondence to Stephen J Meltzer.

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Wang, S., Mori, Y., Sato, F. et al. An LOH and mutational investigation of the ST7 gene locus in human esophageal carcinoma. Oncogene 22, 467–470 (2003). https://doi.org/10.1038/sj.onc.1206125

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