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Microsatellite instability at tetranucleotide repeats in skin and bladder cancer

Oncogene volume 21, pages 4894–4899 (2002)Cite this article

Abstract

Recently, a novel form of MSI has been described that occurs only at tetranucleotide repeat markers. This has been termed elevated microsatellite instability at selected tetranucleotide repeats (EMAST). EMAST has been related to alterations of the p53 gene, and to the nature of the repeat sequence. We initially tested whether loss of heterozygosity (LOH) at the p53 and the patched (ptch) genes was related to EMAST in a series of 61 non-melanoma skin cancer (NMSC) tumors. We then analysed a series of 57 primary bladder cancers for the presence of EMAST, testing whether this was related to mutation or expression of the p53 gene. In both NMSC and bladder tumors we found a high prevalence of EMAST (75.4 and 43.9%). In NMSC the prevalence of EMAST was higher in tumors that had either p53 or ptch LOH, although the difference was not statistically significant. There was a significant association of extensive EMAST (three or more loci) with mutations in p53 among the bladder cancer tumors, but no indication of elevated EMAST in tumors with abnormal p53 staining without mutation. The association of EMAST with p53 mutation was confined to non-invasive disease. Hence, EMAST likely reflects a particular pattern of somatic events that are interactive with p53 mutation, particularly common in skin cancer and limited to non-invasive disease in bladder cancer.

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Acknowledgements

The authors would like to thank Dr Howard Liber, Dr Joseph Paulauskis and Dr John B Little for helpful discussion, and Megan Bronson, Virginia Stannard, Rachel Dow and Christine Hodorowski for technical assistance. Supported by: NIH grants P42ES5947, P42ES07373, CA09078, CA82354, CA57494 and ES00002.

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Authors and Affiliations

  1. Department of Cancer Cell Biology, Harvard School of Public Health, Boston, 02115, Massachusetts, MA, USA

    Hadi Danaee, Heather H Nelson, Tara Devi S Ashok, Tomoko Hirao & Karl T Kelsey

  2. Department of Community and Family Medicine, Dartmouth Medical School, Hanover, 03756, New Hampshire, NH, USA

    Margaret R Karagas

  3. Department of Pathology, Dartmouth Medical School, Hanover, 03756, New Hampshire, NH, USA

    Alan R Schned & Ann E Perry

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  1. Hadi Danaee
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Correspondence to Karl T Kelsey.

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Danaee, H., Nelson, H., Karagas, M. et al. Microsatellite instability at tetranucleotide repeats in skin and bladder cancer. Oncogene 21, 4894–4899 (2002). https://doi.org/10.1038/sj.onc.1205619

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