Abstract
The breast cancer promoting effects of estrogen and the chemopreventive effects of tamoxifen are thought to be mediated by the estrogen receptor, a ligand-dependent transcription factor. Therefore, comprehensive analysis of gene expression profiles following estrogen or tamoxifen treatment may help us better understand the role estrogen plays in tumorigenesis. We utilized SAGE (Serial Analysis of Gene Expression) technology to identify genes regulated by estrogen and tamoxifen in the ZR75-1 estrogen dependent breast cancer cell line. In this manner we have identified several genes that were regulated by estrogen or tamoxifen. Here we report the identification and initial characterization of EIT-6 (Estrogen Induced Tag-6), a novel nuclear protein and a new member of the evolutionarily conserved SM-20 family of growth regulatory immediate-early genes. EIT-6 appears to be a direct transcriptional target of the estrogen receptor and constitutive expression of EIT-6 promotes colony growth in human breast cancer cells. These data indicate that EIT-6 may play a role in estrogen induced cell growth.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Bauer MF, Gempel K, Reichert AS, Rappold GA, Lichtner P, Gerbitz KD, Neupert W, Brunner M, Hofmann S . 1999 J. Mol. Biol. 289: 69–82
Berry LD, Gould KL . 1997 J. Cell. Biol. 137: 1337–1354
Charpentier A, Bednarek A, Daniel R, Hawkins K, Laflin K, Gaddis S, MacLeod M, Aldaz C . 2000 Cancer Res. 60: 5977–5983
de Cupis A, Favoni RE . 1997 Trends Pharmacol. Sci. 18: 245–251
Dupuy D, Aubert I, Duperat VG, Petit J, Taine L, Stef M, Bloch B, Arveiler B . 2000 Genomics 69: 348–354
Eisen MB, Spellman PT, Brown PO, Botstein D . 1998 Proc. Natl. Acad. Sci. USA 95: 14863–14868
Fisher B, Costantino JP, Wickerham DL, Redmond CK, Kavanah M, Cronin WM, Vogel V, Robidoux A, Dimitrov N, Atkins J, Daly M, Wieand S, Tan-Chiu E, Ford L, Wolmark N . 1998 J. Natl. Cancer Inst. 90: 1371–1388
Fisher B, Dignam J, Wolmark N, Wickerham DL, Fisher ER, Mamounas E, Smith R, Begovic M, Dimitrov NV, Margolese RG, Kardinal CG, Kavanah MT, Fehrenbacher L, Oishi RH . 1999 Lancet 353: 1993–2000
Flatt PM, Polyak K, Tang LJ, Scatena CD, Westfall MD, Rubinstein LA, Yu J, Kinzler KW, Vogelstein B, Hill DE, Pietenpol JA . 2000 Cancer Lett. 156: 63–72
Foca C, Rice GE, Quinn MA, Moses EK . 2000 Mol. Hum. Reprod. 6: 712–718
Freedman LP . 1999 Trends Endocrinol. Metab. 10: 403–407
Greenlee RT, Murray T, Bolden S, Wingo PA . 2000 CA Cancer J. Clin. 50: 7–33
He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B . 1998 Proc. Natl. Acad. Sci. USA 95: 2509–2514
Inadera H, Hashimoto S, Dong HY, Suzuki T, Nagai S, Yamashita T, Toyoda N, Matsushima K . 2000 Biochem. Biophys. Res. Commun. 275: 108–114
Ismail PM, Lu T, Sawadogo M . 1999 Oncogene 18: 5582–5591
Katzenellenbogen BS . 1996 Biol. Reprod. 54: 287–293
Katzenellenbogen BS, Montano MM, Ekena K, Herman ME, McInerney EM . 1997 Breast Cancer Res. Treat. 44: 23–38
Lal A, Lash AE, Altschul SF, Velculescu V, Zhang L, McLendon RE, Marra MA, Prange C, Morin PJ, Polyak K, Papadopoulos N, Vogelstein B, Kinzler KW, Strausberg RL, Riggins GJ . 1999 Cancer Res. 59: 5403–5407
Lash AE, Tolstoshev CM, Wagner L, Schuler GD, Strausberg RL, Riggins GJ, Altschul SF . 2000 Genome Res. 10: 1051–1060
Lipscomb EA, Sarmiere PD, Crowder RJ, Freeman RS . 1999 J. Neurochem. 73: 429–432
Madden SL, Galella EA, Riley D, Bertelsen AH, Beaudry GA . 1996 Cancer Res. 56: 5384–5390
Mangelsdorf DJ, Thummel C, Beato M, Herrlich P, Schutz G, Umesono K, Blumberg B, Kastner P, Mark M, Chambon P, Evans R . 1995 Cell 83: 835–839
Manning DL, Archibald LH, Ow KT . 1990 Cancer Res. 50: 4098–4104
Manning DL, Daly RJ, Lord PG, Kelly KF, Green CD . 1988 Mol. Cell. Endocrinol. 59: 205–212
Manning DL, McClelland RA, Knowlden JM, Bryant S, Gee JM, Green CD, Robertson JF, Blamey RW, Sutherland RL, Ormandy CJ, Nicholson RI . 1995 Acta Oncol. 34: 641–646
McMahon C, Suthiphongchai T, DiRenzo J, Ewen ME . 1999 Proc. Natl. Acad. Sci. USA 96: 5382–5387
Moschella MC, Menzies K, Tsao L, Lieb MA, Kohtz JD, Kohtz DS, Taubman MB . 1999 Gene Expr. 8: 59–66
Nakashiro K, Kawamata H, Hino S, Uchida D, Miwa Y, Hamano H, Omotehara F, Yoshida H, Sato M . 1998 Cancer Res. 58: 549–555
Nandi S, Guzman RC, Yang J . 1995 Proc. Natl. Acad. Sci. USA 92: 3650–3657
Perou CM, Sorlie T, Eisen MB, van de Rijn M, Jeffrey SS, Rees CA, Pollack JR, Ross DT, Johnsen H, Akslen LA, Fluge O, Pergamenschikov A, Williams C, Zhu SX, Lonning PE, Borresen-Dale AL, Brown PO, Botstein D . 2000 Nature 406: 747–752
Polyak K, Xia Y, Zweier JL, Kinzler KW, Vogelstein B . 1997 Nature 389: 300–305
Porter D, Krop I, Nasser S, Sgroi D, Kaelin C, Marks J, Riggins G, Polyak K . 2001 Cancer Res. 61: 5697–5702
Rosen ED, Sarraf P, Troy AE, Bradwin G, Moore K, Milstone DS, Spiegelman BM, Mortensen RM . 1999 Mol. Cell. 4: 611–617
Seth P, Lunetta KL, Bell DW, Gray H, Nasser SM, Rhei E, Kaelin CM, Iglehart DJ, Marks JR, Garber JE, Haber DA, Polyak K . 2000 Cancer Res. 60: 6859–6863
Shibanuma M, Kuroki T, Nose K . 1992 J. Biol. Chem. 267: 10219–10224
Trent C, Tsung N, Horvitz R . 1983 Genetics 104: 619–647
Velculescu VE, Zhang L, Vogelstein B, Kinzler KW . 1995 Science 270: 484–487
Wax SD, Rosenfield CL, Taubman MB . 1994 J. Biol. Chem. 269: 13041–13047
Weinshilboum RM, Otterness DM, Aksoy IA, Wood TC, Her C, Raftogianis RB . 1997 FASEB J. 11: 3–14
Xu Q, Reed JC . 1998 Mol. Cell. 1: 337–346
Zheng W, Xie D, Cerhan JR, Sellers TA, Wen W, Folsom AR . 2001 Cancer Epidemiol. Biomarkers Prev. 10: 89–94
Acknowledgements
We thank Carmen Tam and Massimo Loda for mRNA in situ hybridization. Jim DiRenzo and Yongfeng Shang for helpful advice with luciferase assays. Danielle Garneau and Ed Fox for sequencing the SAGE libraries, Bill Sellers, Myles Brown, and Alexander Miron for critical review of the manuscript. This work was, in part, supported by the Sydney Kimmel Cancer Foundation (K Polyak), a US department of Defense postdoctoral fellowship (DAMD17-01-1-0221) to P Seth and the Dana-Farber Cancer Institute.
Accession number The GenBank accession number for the human EIT-6 cDNA sequence reported in this paper is AY040565.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Seth, P., Krop, I., Porter, D. et al. Novel estrogen and tamoxifen induced genes identified by SAGE (Serial Analysis of Gene Expression). Oncogene 21, 836–843 (2002). https://doi.org/10.1038/sj.onc.1205113
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1205113
Keywords
This article is cited by
-
PHD1-3 oxygen sensors in vivo—lessons learned from gene deletions
Pflügers Archiv - European Journal of Physiology (2024)
-
Recent Advances and Therapeutic Implications of 2-Oxoglutarate-Dependent Dioxygenases in Ischemic Stroke
Molecular Neurobiology (2023)
-
Decreased expression of prolyl hydroxylase 1 is associated with poor prognosis in colorectal cancers
Journal of Cancer Research and Clinical Oncology (2023)
-
Cell cycle progression in response to oxygen levels
Cellular and Molecular Life Sciences (2014)
-
Hypoxia Inducible Factor-1: Its Potential Role In Cerebral Ischemia
Cellular and Molecular Neurobiology (2012)