Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

Gastrin induces over-expression of genes involved in human U373 glioblastoma cell migration

Oncogene volume 20, pages 7021–7028 (2001)Cite this article

Abstract

Astrocytic tumors are the most common and the most malignant primary tumors of the central nervous system. We had previously observed that gastrin could significantly modulate both cell proliferation and migration of astrocytoma cells. We have investigated in the present study which genes could be targeted by gastrin in tumor astrocyte migration. Using a subtractive hybridization PCR technique we have cloned genes differentially over-expressed in human astrocytoma U373 cells treated or not with gastrin. We found about 70 genes over-expressed by gastrin. Among the genes overexpressed by gastrin, we paid particular attention to tenascin-C, S100A6 and MLCK genes because their direct involvement in cell migration features. Their gastrin-induced overexpression was quantitatively determined by competitive RT–PCR technique. We also showed by means of a reporter gene system that S100A6 and tenascin-C respective promoters were upregulated after gastrin treatment. These data show that gastrin-mediated effects in glioblastoma cells occur through activation of a number of genes involved in cell migration and suggest that gastrin could be a target in new therapeutic strategies against malignant gliomas.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4

Similar content being viewed by others

References

  • Aspenström P . 1999 Curr. Opin. Cell Biol. 11: 95–102

  • Astruc ME, Chabret C, Bali P, Gagne D, Pons M . 1995 Endocrinology 136: 824–832

  • Burg MA, Nishiyama A, Stallcup WB . 1997 Exp. Cell Res. 235: 254–264

  • Camby I, Belot N, Rorive S, Lefranc F, Maurage CA, Lahm H, Kaltner H, Hadari Y, Ruchoux MM, Brotchi J, Zick Y, Salmon I, Gabius HJ, Kiss R . 2001 Brain Pathol. 11: 12–26

  • Camby I, Nagy N, Lopes MB, Schäfer BW, Maurage CA, Ruchoux MM, Murmann P, Pochet R, Heizmann C, Brotchi J, Salmon I, Kiss R, Decaestecker C . 1999 Brain Pathol. 9: 825–843

  • Camby I, Salmon I, Danguy A, Pasteels JL, Brotchi J, Martinez J, Kiss R . 1996 J. Natl. Cancer Inst. 88: 594–600

  • Camby I, Salmon I, Rorive S, Gras T, Darro F, Kruczynski A, Danguy A, Pasteels JL, Kiss R . 1994 J. Neuro-Oncol. 20: 67–80

  • Chrzanowska-Wodnicka M, Burridge K . 1996 J. Cell Biol. 133: 1403–1415

  • De la Fuente M, Carrasco M, Hernanz A . 1997 J. Endocrinol. 153: 475–483

  • De la Fuente M, Carrasco M, Del Rio M, Hernanz A . 1998 Neuropeptides 32: 225–233

  • Decaestecker C, Camby I, Nagy N, Brotchi J, Kiss R, Salmon I . 1998 Brain Pathol. 8: 29–38

  • DeHauwer C, Camby I, Darro F, Migeotte I, Decaestecker C, Verbeek C, Danguy A, Brotchi J, Salmon I, Van Ham Ph, Kiss R . 1998 J. Neurobiol. 37: 373–382

  • Donato R . 1999 Biophys. Acta. 1450: 191–231

  • Enam AS, Rosenblum ML, Edvardsen K . 1998 Neurosurgery 42: 599–608

  • Gherzi R, Carnemolla B, Siri A, Ponassi M, Balza E, Zardi L . 1995 J. Biol. Chem. 270: 3429–3434

  • Giese A, Kluwe L, Laube B, Meissner H, Berens ME, Westphal M . 1996a Neurosurgery 38: 755–764

  • Giese A, Loo MA, Norman SA, Treasurywala S, Berens ME . 1996b J. Cell Sci. 109: 2161–2168

  • Giese A, Rief MD, Loo MA, Berens ME . 1994 Cancer Res. 54: 3897–3904

  • Gillepsie GY, Soroceanu L, Manning Jr TJ, Gladson CL, Rosenfeld SS . 1999 Cancer Res. 59: 2076–2082

  • Gladson CL . 1999 J. Neuropathol. Exp. Neurol. 58: 1029–1040

  • Goeckeler ZM, Wysolmerski RB . 1995 J. Cell Biol. 130: 613–627

  • Golitsina NL, Kordowska J, Wang CL, Lehrer SS . 1996 Biochem. Biophys. Res. Commun. 220: 360–365

  • Kleihues P, Cavenee WK . 2000 Pathology and genetics of tumours of the nervous system. World Health Organization Classification of Tumours IARC Press: Lyon

    Google Scholar 

  • Klemke RL, Leng J, Molander R, Brooks PC, Vuori K, Cheresh DA . 1998 J. Cell Biol. 140: 961–972

  • Kruczynski A, Salmon I, Camby I, Limouzy A, Rombaut K, Pasteels JL, Delsol G, Brotchi J, Kiss R . 1995 Int. J. Oncol. 6: 473–481

  • Lamb NJ, Fernandez A, Conti MA, Adelstein R, Glass DB, Welch WJ, Feramisco JR . 1988 J. Cell Biol. 106: 1955–1971

  • Lauffenburger DA, Horwitz AF . 1996 Cell 84: 359–369

  • Legler JM, Gloeckler-Ries LA, Smith MA, Warren JL, Heineman EF, Kaplan RS, Linet MS . 1999 J. Natl. Cancer Inst. 91: 1382–1390

  • Machesky LM, Mullins RD, Higgs HN, Kaiser DA, Blanchoin L, May RC, Hall ME, Pollard TD . 1999 Proc. Natl. Acad. Sci. USA 96: 3739–3744

  • Maekawa M, Ishizaki T, Boku S, Watanabe N, Fujita A, Iwamatsu A, Obinata T, Ohashi K, Mizuno K, Narumiya S . 1999 Science 285: 895–898

  • Mettouchi A, Cabon F, Montreau N, Dejong V, Vernier P, Gherzi R, Mercier G, Binétruy B . 1997 Mol. Cell. Biol. 17: 3202–3209

  • Rehfeld JF, Van Solinge WW . 1991 Adv. Cancer Res. 63: 295–347

  • Sasaki H, Yoshida K, Ikeda E, Asou H, Inaba M, Otani M, Kawase T . 1998 Cancer 82: 1921–1931

  • Schäfer BW, Heizmann CW . 1996 Trends in Biochem. Sci. 21: 134–140

  • Seufferlein T, Withers DJ, Broad S, Hervet T, Walsh JH, Rozengurt E . 1995 Cell Growth Differ. 6: 383–393

  • Stepan VM, Dickinson CJ, Del Valle J, Matsushima M, Todisco A . 1999a Am. J. Physiol. 276: G1363–G1372

  • Stepan VM, Tatewaki M, Matsushima M, Dickinson CJ, Del Valle J, Todisco A . 1999b Am. J. Physiol. 276: G417–G424

  • Takenaga K, Nakamura Y, Sakiyama S . 1994 Cell Struct. Funct. 19: 133–141

  • Taniguchi T, Takaishi K, Murayama T, Ito M, Iwata N, Chihara K, Sasaki T, Takai Y, Matsui T . 1996 Oncogene 12: 1357–1360

  • Tapon N, Hall A . 1997 Curr. Opin. Cell Biol. 9: 86–92

  • Treasurywala S, Berens ME . 1998 GLIA 24: 236–243

  • Vande Heuvel JP, Tyson FL, Bell DA . 1993 Biotechniques 14: 395–398

  • Waterman-Storer CM, Salmon ED . 1999 Curr. Opin. Cell Biol. 11: 61–67

  • Zhang H, Kelly G, Zerillo C, Jaworski DM, Hockfield S . 1998 J. Neurosci. 18: 2370–2376

Download references

Acknowledgements

We gratefully thank Dr X Tu (T Jefferson University, Philadelphia, PA, USA) for providing p2A9-CAT vectors. We are also grateful to Dr B Binétruy (University of Nice Sophia-Antipolis, Nice, France) for tenascin-C CAT constructs and Dr R Chiquet (Friedrich Miescher Institute, Basel, Switzerland) for tenascin-C luciferase construct. The present work has been carried out on the basis of grants awarded by the Centre National de Recherche Scientifique (CNRS, France), by the Ministère de l'Enseignement Supérieur et de la Recherche and by the Fonds National de la Recherche Scientifique (FNRS, Belgium). C Decaestecker and R Kiss are a Research Associate and a Senior Research Associate with the FNRS. I Camby is the holder of a grant from the Rose & Jean Hoguet Foundation.

Author information

Authors and Affiliations

  1. Laboratoire des Amino Acides, Peptides et Protéines (L.A.P.P) UMR CNRS 5810, Universités Montpellier I et II, Faculté de Pharmacie, 15 Av. C. Flahault, Montpellier, 34060, France

    Jérôme Kucharczak, Julie Pannequin & Jean Martinez

  2. Laboratoire d'Histopathologie, Faculté de Médecine, Université Libre de Bruxelles, Belgium

    Isabelle Camby, Christine Decaestecker & Robert Kiss

Authors
  1. Jérôme Kucharczak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Julie Pannequin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Isabelle Camby
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Christine Decaestecker
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Robert Kiss
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jean Martinez
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jean Martinez.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kucharczak, J., Pannequin, J., Camby, I. et al. Gastrin induces over-expression of genes involved in human U373 glioblastoma cell migration. Oncogene 20, 7021–7028 (2001). https://doi.org/10.1038/sj.onc.1204882

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1204882

Keywords

This article is cited by

Search

Advanced search

Quick links