Abstract
The murine Unp gene encodes a widely expressed ubiquitin-specific protease. The predicted sequence of the UNP protein features motifs common to viral oncoproteins through which these proteins interact with the retinoblastoma gene product pRb, as well as the related ‘pocket proteins’ p107 and p130. We have explored the possibility that UNP interacts with pocket proteins, and report here that such associations can be detected in vitro and in cells. Associations of UNP and pocket proteins are sensitive to site-directed mutations in a manner directly analogous to those documented in viral oncoproteins. We conclude that within cells UNP does physically associate with pRb, and can also associate with p107 and p130.
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References
Beijersbergen RL, Hijmans EM, Zhu L, Bernards R . 1994 EMBO J. 13: 4080–4086
Boyer SN, Wazer DE, Band V . 1996 Cancer Res. 56: 4620–4624
Buyse IM, Shao G, Huang S . 1995 Proc. Natl. Acad. Sci. USA 92: 4467–4471
Cadavid AL, Ginzel A, Fischer JA . 2000 Development 127: 1727–1736
Carrano AC, Eytan E, Hershko A, Pagano M . 1999 Nat. Cell. Biol. 1: 193–199
DeCaprio JA, Ludlow JW, Figge J, Shew JY, Huang CM, Lee WH, Marsilio E, Paucha E, Livingston DM . 1988 Cell 54: 275–283
DeSalle LM, Latres E, Lin D, Graner E, Montagnoli A, Baker RT, Pagano M, Loda M . 2001 Oncogene 20: 5538–5542
Diehl JA, Zindy F, Sherr CJ . 1997 Genes Dev. 11: 957–972
Dyson N, Howley PM, Munger K, Harlow E . 1989 Science 243: 934–937
Fattaey AR, Helin K, Dembski MS, Dyson N, Harlow E, Vuocolo GA, Hanobik MG, Haskell KM, Oliff A, Defeo-Jones D, Jones RE . 1993 Oncogene 8: 3149–3156
Ginsberg D, Vairo G, Chittenden T, Xiao ZX, Xu G, Wydner KL, DeCaprio JA, Lawrence JB, Livingston DM . 1994 Genes Dev. 8: 2665–2679
Glotzer M, Murray AW, Kirschner MW . 1991 Nature 349: 132–138
Grana X, Garriga J, Mayol X . 1998 Oncogene 17: 3365–3383
Gray DA, Inazawa J, Gupta K, Wong A, Ueda R, Takahashi T . 1995 Oncogene 10: 2179–2183
Gregory MA, Hann SR . 2000 Mol. Cell. Biol. 20: 2423–2435
Gupta K, Chevrette M, Gray DA . 1994 Oncogene 9: 1729–1731
Hateboer G, Kerkhoven RM, Shvarts A, Bernards R, Beijersbergen RL . 1996 Genes Dev. 10: 2960–2970
Helin K, Lees JA, Vidal M, Dyson N, Harlow E, Fattaey A . 1992 Cell 70: 337–350
Hofmann F, Martelli F, Livingston DM, Wang Z . 1996 Genes Dev. 10: 2949–2959
Hoyt MA . 1997 Cell 91: 149–151
Koepp DM, Harper JW, Elledge SJ . 1999 Cell 97: 431–434
LeCouter JE, Kablar B, Hardy WR, Ying C, Megeney LA, May LL, Rudnicki MA . 1998a Mol. Cell. Biol. 18: 7455–7465
LeCouter JE, Kablar B, Whyte PF, Ying C, Rudnicki MA . 1998b Development 125: 4669–4679
Lipinski MM, Jacks T . 1999 Oncogene 18: 7873–7882
Nevins JR . 1998 Cell Growth Differ. 9: 585–593
Sala A, De Luca A, Giordano A, Peschle C . 1996 J. Biol. Chem. 271: 28738–28740
Shan B, Zhu X, Chen PL, Durfee T, Yang Y, Sharp D, Lee WH . 1992 Mol. Cell Biol. 12: 5620–5631
Smith EJ, Leone G, Nevins JR . 1998 Cell Growth Differ. 9: 297–303
Voorhoeve PM, Hijmans EM, Bernards R . 1999 Oncogene 18: 515–524
Whyte P, Williamson NM, Harlow E . 1989 Cell 56: 67–75
Acknowledgements
This work was supported by National Cancer Institute of Canada grant number 010068, awarded to DA Gray, and by a studentship from the National Science and Engineering Research Council of Canada to P Blanchette. We are indebted to Dr Michael Rudnicki for the gift of lysates from p107 and p130 null mice.
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Blanchette, P., Gilchrist, C., Baker, R. et al. Association of UNP, a ubiquitin-specific protease, with the pocket proteins pRb, p107 and p130. Oncogene 20, 5533–5537 (2001). https://doi.org/10.1038/sj.onc.1204823
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DOI: https://doi.org/10.1038/sj.onc.1204823
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