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  • Oncogenomics
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Chromosomal localization and promoter analysis of the adenomatous polyposis coli binding protein RP1

Oncogene volume 20, pages 5920–5929 (2001)Cite this article

Abstract

The EB1/RP1 family is a new protein family that is characterized by the ability of its members to serve as interacting partners for the adenomatous polyposis coli (APC) tumour suppressor protein and tubulin. Data obtained with highly conserved yeast homologues suggest that the EB1/RP1 protein family promotes cytoplasmic microtubule dynamics and contributes to the sensor mechanism controlling the cytokinesis checkpoint during mitosis. However, the precise function of this protein family in mammalian cells has not been elucidated so far and remains unclear. Here, we report on the genomic localization of the RP1 gene and the characterization of the corresponding promoter. The RP1 gene was found to be encoded on chromosome 18q21, a locus which is altered or deleted in up to 50% of all patients with colorectal cancer. Promoter analysis revealed that the RP1 gene is under the control of a strong promoter that was 10 times more active in mammalian cells when compared to SV40 promoter. Members of the cyclic AMP response element binding protein family (CREB1 and CREB2) could be identified as transcription factors binding specifically within the RP1 promoter sequence.

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References

  • Beier F, Lee RJ, Taylor AC, Pestell RG, LuValle P . 1999 Proc. Natl. Acad. Sci. USA 96: 1433–1438

  • Beinhauer JD, Hagan IM, Hegemann JH, Fleig U . 1997 J. Cell Biol. 139: 717–728

  • Bodmer W, Bailey C, Bodmer J, Bussey H, Ellis A, Gorman P, Lucibello F, Murday V, Rider S, Scambler P . 1987 Nature 328: 614–616

  • Butscher WG, Powers C, Olive M, Vinson C, Gardner K . 1998 J. Biol. Chem. 273: 552–560

  • Cibelli G, Jüngling S, Schoch S, Gerdes HH, Thiel G . 1996 Eur. J. Biochem. 236: 171–179

  • Cibelli G, Schoch S, Thiel G . 1999 Eur. J. Cell Biol. 78: 642–649

  • Drubin DG, Nelson WJ . 1996 Cell 84: 335–344

  • Feuerstein N, Huang D, Hinrichs SH, Orten DJ, Aiyar N, Prystowsky MB . 1995 J. Immunol. 54: 68–79

  • Groden J, Thliveris A, Samovitz W, Carlson M, Lawrence G, Albertsen H, Joslyn G, Stevens J, Spirio L, Roberson M . 1991 Cell 66: 589–600

  • Hsueh YP, Liang HE, Ng SY, Lai MZ . 1997 J. Immunol. 158: 85–93

  • Jernvall P, Makinen MJ, Karttunen TJ, Makela J, Vihko P . 1999 Br. J. Cancer 79: 903–908

  • Juwana JP, Henderix P, Mischo A, Wadle A, Fadle N, Gerlach K, Arends JW, Hoogenboom H, Pfreundschuh M, Renner C . 1999 Int. J. Cancer 81: 275–284

  • Kinzler KW, Vogelstein B . 1996 Cell 87: 159–170

  • Korinek WS, Copeland MJ, Chaudhuri A, Chant J . 2000 Science 287: 2257–2259

  • Muhua L, Adames NR, Murphy MD, Shields CR, Cooper JA . 1998 Nature 393: 487–491

  • Munemitsu S, Souza B, Müller O, Albert I, Rubinfeld B, Polakis P . 1994 Cancer Res. 54: 3676–3681

  • Näthke IS, Adams CL, Polakis P, Sellin JH, Nelson WJ . 1996 J. Cell Biol. 134: 165–179

  • Ogunbiyi OA, Goodfellow PJ, Herfarth K, Gagliardi G, Swanson PE, Birnbaum EH, Read TE, Fleshman JW, Kodner IJ, Moley JF . 1998 J. Clin. Onco. 16: 427–433

  • Papadimitrakopoulou VA, Oh Y, El-Naggar A, Izzo J, Clayman G, Mao L . 1998 Clin. Cancer Res. 4: 539–544

  • Polakis P . 1997 Biochim. Biophys. Acta 1332: 127–147

  • Quandt K, Frech K, Karas H, Wingender E, Werner T . 1995 Nucleic Acids Res. 23: 4878–4884

  • Renner C, Pfitzenmeier JP, Gerlach K, Held G, Ohnesorge S, Sahin U, Bauer St, Pfreundschuh M . 1997 J. Immunol. 159: 1276–1283

  • Rubinfeld B, Souza B, Albert I, Müller O, Chamberlain SH, Masiarz FR, Munemitsu S, Polakis P . 1993 Science 262: 1731–1733

  • Sambrook J, Fritsch EF, Maniatis T. (eds) . 1989 A Laboratory Manual 2nd Edn Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, USA

    Google Scholar 

  • Schwartz K, Richards K, Botstein D . 1997 Mol. Biol. Cell 8: 2677–2691

  • Smith DB, Johnson KS . 1988 Gene 67: 31–40

  • Takemaru KI, Moon RT . 2000 J. Cell Biol. 149: 249–254

  • Tarafa G, Villanueva A, Farre L, Rodriguez J, Musulen E, Reyes G, Seminago R, Olmedo E, Paules AB, Peinado MA, Bachs O, Capella G . 2000 Oncogene 19: 546–555

  • Tetsu O, McCormick F . 1999 Nature 398: 422–426

  • Thiel G, Petersohn D, Schoch S . 1996 Gene 168: 173–176

  • Tirnauer JS, O'Toole E, Berrueta L, Bierer BE, Pellman D . 1999 J. Cell Biol. 145: 993–1007

  • Van der Luijt R, Meera-Khan P, Vasen H, van Leeuwen C, Tops C, Roest P, den Dunnen J, Fodde R . 1994 Genomics 29: 1–4

  • Zhang Y, Matthiesen P, Siebert R, Harder S, Theile M, Scherneck S, Schlegelberger B . 1998 Hum. Genet. 103: 727–729

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Acknowledgements

We thank Gabi Carbon, Natalie Fadle and Birgit Bette for excellent technical assistance. The study was supported in part by grant Re 1271/1-1 and grant Re 1271/1-2 from the Deutsche Forschungsgemeinschaft.

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Authors and Affiliations

  1. Med. Department I, Saarland University Medical School, Homburg, D-66421, Germany

    Andreas Wadle, Axel Mischo, Michael Pfreundschuh & Christoph Renner

  2. Medical Biochemistry and Molecular Biology, Saarland University Medical School, Homburg, D-66421, Germany

    Gerald Thiel

  3. Institute for Human Genetics, Saarland University Medical School, Homburg, D-66421, Germany

    Volker Jung

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  1. Andreas Wadle
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  2. Gerald Thiel
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  3. Axel Mischo
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  6. Christoph Renner
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Correspondence to Christoph Renner.

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Wadle, A., Thiel, G., Mischo, A. et al. Chromosomal localization and promoter analysis of the adenomatous polyposis coli binding protein RP1. Oncogene 20, 5920–5929 (2001). https://doi.org/10.1038/sj.onc.1204797

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