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Sporadic testicular germ cell cancers do not exhibit specific alteration in CAG/CTG repeats containing genes expressed in human testis

Oncogene volume 20, pages 5548–5553 (2001)Cite this article

Abstract

CAG/CTG repeat expansions in genomic DNA of testicular tumour cell lines, and germline DNA from members of families predisposed to this malignancy, have been previously described. In order to identify genes possibly concerned by this alteration, we attempted to clone all possible human testis cDNA containing at least five CAG/CTG repeats. Thirty-four different transcripts were identified. By using PCR and non denaturing gel electrophoresis, we determined the size of their repeats, as well as their polymorphisms in a collection of human testicular germ cell tumours and the normal surrounding tissues. For all tested genes, we detected the presence of several species of the same mRNA for each person. Nine genes exhibited specific patterns of expression among different groups of individuals, indicative of polymorphism. None of these polymorphisms was related to human testicular tumours.

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Acknowledgements

We thank Y Laperche for helpful discussions. This work was funded by INSERM.

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Authors and Affiliations

  1. Unité 99 INSERM, Hôpital Henri Mondor, Créteil, 94010, France

    Yasumasa Ono, Georges Guellaën & Frédérique Bulle

  2. Copenhagen University, Dept of Growth and Reproduction, Rigshospitalet, Copenhagen, DK-2100, Denmark

    Eva Rajpert De-Meyts

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  1. Yasumasa Ono
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  2. Eva Rajpert De-Meyts
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  4. Frédérique Bulle
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Correspondence to Frédérique Bulle.

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Ono, Y., De-Meyts, E., Guellaën, G. et al. Sporadic testicular germ cell cancers do not exhibit specific alteration in CAG/CTG repeats containing genes expressed in human testis. Oncogene 20, 5548–5553 (2001). https://doi.org/10.1038/sj.onc.1204714

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