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The Brn-3a transcription factor plays a key role in regulating the growth of cervical cancer cells in vivo

Oncogene volume 20, pages 4899–4903 (2001)Cite this article

Abstract

The cellular Brn-3a transcription factor is known to activate transcription of the genes encoding the human papilloma virus E6 and E7 proteins and is over-expressed in women with cervical neoplasia. We show that cervical cell lines with reduced Brn-3a expression show a greatly reduced ability to form tumours in nude mice compared to control cells and also show reduced expression of the HPV E6 and cellular Bcl-2 oncogenes. These effects are also observed in cervical cells over-expressing the related Brn-3b factor, which is known to antagonize activation of HPV gene expression by Brn-3a. These results demonstrate, for the first time, that inhibition of Brn-3a expression or enhanced Brn-3b expression can inhibit cervical cell-derived tumour growth in vivo as well as in vitro. Hence they establish Brn-3a as a key factor in cervical tumorigenesis and as a potential therapeutic target in human cervical neoplasia.

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Acknowledgements

This work was supported by the Association for International Cancer Research (AICR) and WellBeing (Royal College of Obstetricians and Gynaecologists).

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Authors and Affiliations

  1. Medical Molecular Biology Unit, Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK

    Daniel Ndisang, Vishwanie Budhram-Mahadeo & David S Latchman

  2. Department of Oncology, Royal Free and University College Medical School, Rowland Hill Street, London, NW3, UK

    Barbara Pedley

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  1. Daniel Ndisang
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  2. Vishwanie Budhram-Mahadeo
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  3. Barbara Pedley
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  4. David S Latchman
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Ndisang, D., Budhram-Mahadeo, V., Pedley, B. et al. The Brn-3a transcription factor plays a key role in regulating the growth of cervical cancer cells in vivo. Oncogene 20, 4899–4903 (2001). https://doi.org/10.1038/sj.onc.1204634

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