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Expression of truncated FHIT transcripts in cervical cancers and in normal human cells

Oncogene volume 20, pages 4665–4675 (2001)Cite this article

Abstract

To analyse FHIT transcription patterns in cervical cancer, a series of primary cervical tumors and normal control samples were studied using RT–PCR. Full length and truncated FHIT transcripts were detectable in all samples tested. Interestingly, the expression of truncated FHIT transcripts by primary epithelial cells in vitro was associated with confluency. The breakpoints of most transcript deletions coincided with genuine splice site sequences, suggesting that they resulted from alternative splicing. These findings demonstrate that truncated FHIT transcripts are commonly detected in both normal and tumor tissues, and suggest that these altered transcripts are not causally related to tumorigenesis in cervical cancer.

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Acknowledgements

We thank Jennifer Koop and Hadar Sheffer for their excellent technical assistance, and the presented work was supported by NIH grant CA42792.

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Authors and Affiliations

  1. Fred Hutchinson Cancer Research Center, Cancer Biology Program, 1100 Fairview Avenue N., P.O. Box 19024, Seattle, 98109-1024, Washington, WA, USA

    C P Matthews, K Shera & J K McDougall

  2. Department of Pathology, University of Washington, Box 357470, 1959 Pacific Street N.E., Seattle, 98195-7407, Washington, WA, USA

    C P Matthews, N Kiviat & J K McDougall

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  1. C P Matthews
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  2. K Shera
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Correspondence to C P Matthews.

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Matthews, C., Shera, K., Kiviat, N. et al. Expression of truncated FHIT transcripts in cervical cancers and in normal human cells. Oncogene 20, 4665–4675 (2001). https://doi.org/10.1038/sj.onc.1204622

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