Abstract
A number of non-virally and non-chemically immortalized chicken embryo fibroblast (CEF) cells have been established recently in continuous cell culture. All immortal CEF cells tested showed common genetic alterations in the expression patterns of p53 and E2F-1 mRNA and protein which were down- and up-regulated, respectively. The biological effects of differentially regulated p53 and E2F-1 were determined by reporter gene transcriptional activity assays, DNA binding assays, and Northern blot analysis of the expression patterns of down-stream genes. In addition, expression of most of the cyclin genes was up-regulated in immortal CEF cells, which may be associated with the rapid cell division rates and serum-independent growth patterns seen in immortal CEF cells. The telomeric lengths and chromosome integrity were maintained in all immortal CEF cell lines without detectable telomerase activity. Although the functional inactivations of the p53 and Rb regulatory pathways are known to be common events for cellular immortalization, the genetic changes leading to alteration of p53 and E2F-1 function through transcriptional and post-transcriptional regulation seem to be unique in immortal CEF cells.
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References
Atadja P, Wong H, Garkavtsev I, Veillette C, Riabowol K . 1995 Proc. Natl. Acad. Sci. USA 92: 8348–8352
Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S, Wright WE . 1998 Science 279: 349–352
Boulton SJ, Jackson SP . 1996 Nucleic Acids Res. 24: 4639–4648
Bryan TM, Englezou A, Gupta J, Bacchetti S, Reddel RR . 1995 EMBO J. 14: 4240–4248
Bryan TM, Marusic L, Bacchetti S, Namba M, Reddel RR . 1997 Hum. Mol. Genet. 6: 921–926
Chen X, Farmer G, Zhu H, Prywes R, Prives C . 1993 Genes Dev. 7: 1837–1849
Dimri GP, Lee X, Basile G, Acosta M, Sscott G, Roskelley D, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O, Peacocke M, Campisi J . 1995 Proc. Natl. Acad. Sci. USA 92: 9363–9367
Dyson N . 1998 Genes Dev. 12: 2245–2262
Giaccia AJ, Kastan MB . 1998 Genes Dev. 12: 2973–2983
Greenwell PW, Kronmal SL, Porter SE, Gassenhuber J, Obermaier B, Petes TD . 1995 Cell 82: 823–829
Hahn WC, Counter CM, Lundberg AS, Beijersbergen RL, Brooks MW, Weinberg RA . 1999 Nature 400: 464–468
Harley CB, Futcher AB, Greider CW . 1990 Nature 345: 458–460
Haupt Y, Maya R, Kazaz A, Oren M . 1997 Nature 387: 296–299
Hayflick L . 1965 Exp. Cell Res. 37: 614–636
Helin K . 1998 Curr. Opin. Genet. Dev. 8: 28–35
Himly M, Foster DN, Bottoli I, Lacovoni JS, Vogt PK . 1998 Virology 248: 295–304
Jha KK, Banga S, Palejwala BV, Ozer HL . 1998 Exp. Cell Res. 245: 1–7
Kaelin WC . 1999 BioEssays 21: 950–958
Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, Coviello GM, Wright WE, Weinrich SL, Shay JW . 1994 Science 266: 2011–2015
Ko LJ, Prives C . 1996 Genes Dev. 10: 1054–1072
Levine AJ . 1997 Cell 88: 323–331
McEachern MJ, Blackburn EH . 1996 Genes Dev. 10: 1822–1834
Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, Ziaugra L, Beijersbergen RL, Davidoff MJ, Liu Q, Bacchetti S, Haber DA, Weinberg RA . 1997 Cell 90: 785–795
Meyyappan M, Wong H, Hull C, Riabowol KT . 1998 Mol. Cell. Biol. 18: 3163–3172
Ponce de León FA, Li Y, Weng Z . 1992 J. Hered. 83: 36–42
Robertson KD, Jones PA . 1998 Mol. Cell. Biol. 18: 6457–6473
Rubelj I, Pereira-Smith OM . 1994 Exp. Cell Res. 211: 82–89
Schaefer-Klein J, Gibol I, Barsov EV, Whitcomb JM, VanBrocklin M, Foster DN, Federspiel MJ, Hughes SH . 1998 Virology 248: 305–311
Scheffner M, Werness BA, Huibregtse JM, Levine AJ, Howley PM . 1990 Cell 63: 1129–1136
Sedivy JM . 1998 Proc. Natl. Acad. Sci. USA 95: 9078–9081
Segawa K, Minowa A, Sugasawa K, Takano T, Hanaoka F . 1993 Oncogene 8: 543–548
Shay JW, Pereira-Smith OM, Wright WE . 1991 Exp. Cell Res. 196: 33–39
Sherr CJ . 1996 Science 274: 1672–1677
Venkatesan RN, Price C . 1998 Proc. Natl. Acad. Sci. USA 95: 14763–14768
Williams BO, Remington L, Albert DM, Mukai S, Bronson RT, Jacks T . 1994 Nature Genet. 7: 480–484
Zhang HS, Postigo AA, Dean DC . 1999 Cell 97: 53–61
Acknowledgements
We thank Dr Charles J Sherr (St Jude Children's Research Hospital) for providing the chicken cyclin cDNAs. This work was supported, in part, by USDA/NRICGP grant #9603280 and a grant from American Home Products (Fort Dodge Animal Health) to DN Foster.
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Kim, H., You, S., Kim, IJ. et al. Alterations in p53 and E2F-1 function common to immortalized chicken embryo fibroblasts. Oncogene 20, 2671–2682 (2001). https://doi.org/10.1038/sj.onc.1204378
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DOI: https://doi.org/10.1038/sj.onc.1204378
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