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Molecular characterization of prostate carcinoma tumor antigen-1, PCTA-1, a human Galectin-8 related gene

Oncogene volume 19, pages 4405–4416 (2000)Cite this article

Abstract

The galectin family of proteins has been associated with several diverse cellular processes. More than 30 years since the discovery of the first member, precise biological functions for the family as a whole, or for individual members has proven elusive. The isolation of Prostate Carcinoma Tumor Antigen-1 (PCTA-1), a cDNA closely related to rat and human Galectin-8, as a surface marker associated with prostate cancer was achieved using a previously described immunological subtraction approach, Surface Epitope Masking (SEM) approach, in combination with expression screening. It appears that PCTA-1 expression is almost ubiquitous in normal human tissues and could alter in specific contexts such as transformation or metastasis. Multiple expression isoforms of PCTA-1 at the mRNA level are observed. PCTA-1 maps to 1q42-43, a locus associated with predisposition to prostate cancer. We have determined the genomic structure of PCTA-1 to account for the several observed isoforms, performed expression analysis to determine distribution in normal and transformed contexts at the RNA and protein level and conducted over-expression studies to determine effects on cellular phenotype.

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References

  • Akahani S, Nangia-Makker P, Inohara H, Kim HR and Raz A. . 1997 Cancer Res. 57: 5272–5276.

  • Arata Y, Hirabayashi J and Kasai K. . 1997 J. Biochem. (Tokyo) 121: 1002–1009.

  • Barondes SH, Cooper DN, Gitt MA and Leffler H. . 1994 J. Biol. Chem. 269: 20807–20810.

  • Bassen R, Brichory F, Caulet-Maugendre S, Bidon N, Delaval P, Desrues B and Dazord L. . 1999 Anticancer Res. 19: 5429–5433.

  • Berthon P, Valeri A, Cohen-Akenine A, Drelon E, Paiss T, Wohr G, Latil A, Millasseau P, Mellah I, Cohen N, Blanche H, Bellane-Chantelot C, Demenais F, Teillac P, Le Duc A, de Petriconi R, Hautmann R, Chumakov I, Bachner L, Maitland NJ, Lidereau R, Vogel W, Fournier G, Mangin P and Cussenot O et al. 1998 Am. J. Hum. Genet. 62: 1416–1424.

  • Blaser C, Kaufmann M, Muller C, Zimmermann C, Wells V, Mallucci L and Pircher H. . 1998 Eur. J. Immunol. 28: 2311–2319.

  • Bresalier RS, Byrd JC, Wang L and Raz A. . 1996 Cancer Res. 56: 4354–4357.

  • Bresalier RS, Yan PS, Byrd JC, Lotan R and Raz A. . 1997 Cancer 80: 776–787.

  • Chadli A, LeCaer JP, Bladier D, Joubert-Caron R and Caron M. . 1997 J. Neurochem. 68: 1640–1647.

  • Chammas R, Jasiulionis MG, Ventura AM, Travassos LR and Brentani RR. . 1996 Braz. J. Med. Biol. Res. 29: 1141–1149.

  • Cho M and Cummings RD. . 1995a J. Biol. Chem. 270: 5198–5206.

  • Cho M and Cummings RD. . 1995b J. Biol. Chem. 270: 5207–5212.

  • Colnot C, Ripoche MA, Scaerou F, Foulis D and Poirier F. . 1996 Biochem. Soc. Trans. 24: 141–146.

  • Cooper DN and Barondes SH. . 1999 Glycobiology 9: 979–984.

  • Cooper DN, Boulianne RP, Charlton S, Farrell EM, Sucher A and Lu BC. . 1997 J. Biol. Chem. 272: 1514–1521.

  • Cortegano I, del Pozo V, Cardaba B, de Andres B, Gallardo S, del Amo A, Arrieta I, Jurado A, Palomino P, Liu FT and Lahoz C. . 1998 J. Immunol. 161: 385–389.

  • Cox DR, Burmeister M, Price ER, Kim S and Myers RM. . 1990 Science 250: 245–250.

  • Dagher SF, Wang JL and Patterson RJ. . 1995 Proc. Natl. Acad. Sci. USA 92: 1213–1217.

  • Ellerhorst J, Nguyen T, Cooper DN, Lotan D and Lotan R. . 1999 Int. J. Oncol. 14: 217–224.

  • Feichtinger W and Schmid M. . 1989 Hum. Genet. 83: 145–147.

  • Feinstein E, Druck T, Kastury K, Berissi H, Goodart SA, Overhauser J, Kimchi A and Huebner K. . 1995 Genomics 29: 305–307.

  • Gillenwater A, Xu XC, el-Naggar AK, Clayman GL and Lotan R. . 1996 Head Neck 18: 422–432.

  • Gitt MA, Colnot C, Poirier F, Nani KJ, Barondes SH and Leffler H. . 1998a J. Biol. Chem. 273: 2954–2960.

  • Gitt MA, Wiser MF, Leffler H, Herrmann J, Xia YR, Massa SM, Cooper DN, Lusis AJ and Barondes SH. . 1995 J. Biol. Chem. 270: 5032–5038.

  • Gitt MA, Xia YR, Atchison RE, Lusis AJ, Barondes SH and Leffler H. . 1998b J. Biol. Chem. 273: 2961–2970.

  • Gopalkrishnan RV, Christiansen KA, Goldstein NI, DePinho RA and Fisher PB. . 1999 Nucleic Acids Res. 27: 4775–4782.

  • Gopalkrishnan RV, Dolle P, Mattei MG, La Thangue NB and Kedinger C. . 1996 Oncogene 13: 2671–2680.

  • Greenhalgh CJ, Beckham SA and Newton SE. . 1999 Mol. Biochem. Parasitol. 98: 285–289.

  • Hadari YR, Paz K, Dekel R, Mestrovic T, Accili D and Zick Y. . 1995 J. Biol. Chem. 270: 3447–3453.

  • Hadj Sahraoui Y, Seve AP, Doyennette-Moyne MA, Saffar L, Felin M, Aubery M, Gattegno L and Hubert J. . 1996 J. Cell. Biochem. 62: 529–542.

  • Hebert E, Roche AC, Nachtigal M and Monsigny M. . 1996 CR Acad. Sci. III 319: 871–877.

  • Hsu DK, Dowling CA, Jeng KC, Chen JT, Yang RY and Liu FT. . 1999 Int. J. Cancer 81: 519–526.

  • Hughes RC. . 1997 Biochem. Soc. Trans. 25: 1194–1198.

  • Iglesias MM, Rabinovich GA, Ambrosio AL, Castagna LF, Sotomayor CE and Wolfenstein-Todel C. . 1998 Glycobiology 8: 59–65.

  • Inohara H and Raz A. . 1995 Cancer Res. 55: 3267–3271.

  • Jiang H, Su ZZ, Lin JJ, Goldstein NI, Young CS and Fisher PB. . 1996 Proc. Natl. Acad. Sci. USA 93: 9160–9165.

  • Kaltner H and Stierstorfer B. . 1998 Acta Anat. 161: 162–179.

  • Kasai K and Hirabayashi J. . 1996 J. Biochem. (Tokyo) 119: 1–8.

  • Li YS, Ramsay DA, Fan YS, Armstrong RF and Del Maestro RF. . 1995 Cancer Genet Cytogenet. 84: 46–50.

  • Lotan R, Belloni PN, Tressler RJ, Lotan D, Xu XC and Nicolson GL. . 1994 Glycoconj J. 11: 462–468.

  • Lu Y, Amos B, Cruise E, Lotan D and Lotan R. . 1998 Biol. Chem. 379: 1323–1331.

  • Lu Y and Lotan R. . 1999 Biochim. Biophys. Acta 1444: 85–91.

  • Lutomski D, Fouillit M, Bourin P, Mellottee D, Denize N, Pontet M, Bladier D, Caron M and Joubert-Caron R. . 1997 Glycobiology 7: 1193–1199.

  • Maldonado CA, Castagna LF, Rabinovich GA and Landa CA. . 1999 Invest. Ophthalmol. Vis. Sci. 40: 2971–2977.

  • Maquoi E, van den Brule FA, Castronovo V and Foidart JM. . 1997 Placenta 18: 433–439.

  • Mehul B and Hughes RC. . 1997 J. Cell. Sci. 110: 1169–1178.

  • Murty VV, Li RG, Mathew S, Reuter VE, Bronson DL, Bosl GJ and Chaganti RS. . 1994 Cancer Res. 54: 3983–3985.

  • Perillo NL, Pace KE, Seilhamer JJ and Baurn LG. . 1995 Nature 378: 736–739.

  • Puch S and Bhavanandan VP. . 1999 Urology 53: 848–852.

  • Remmelink M, Darro F, Decaestecker C, De Decker R, Bovin NV, Gebhart M, Kaltner H, Gabius HJ, Kiss R, Salmon I and Danguy A. . 1999 J. Cancer Res. Clin. Oncol. 125: 275–285.

  • Rini JM. . 1995 Curr. Opin. Struct. Biol. 5: 617–621.

  • Sarafian V, Jadot M, Foidart JM, Letesson JJ, Van den Brule F, Castronovo V, Wattiaux R and Coninck SW. . 1998 Int. J. Cancer 75: 105–111.

  • Schoeppner HL, Raz A, Ho SB and Bresalier RS. . 1995 Cancer 75: 2818–2826.

  • Shen R, Su ZZ, Olsson CA, Goldstein NI and Fisher PB. . 1994 J. Natl. Cancer Inst. 86: 91–98.

  • Su ZZ, Lin J, Shen R, Fisher PE, Goldstein NI and Fisher PB. . 1996 Proc. Natl. Acad. Sci. USA. 93: 7252–7257.

  • Su ZZ, Madireddi MT, Lin JJ, Young CS, Kitada S, Reed JC, Goldstein NI and Fisher PB. . 1998 Proc. Natl. Acad. Sci. USA 95: 14400–14405.

  • Vyakarnam A, Dagher SF, Wang JL and Patterson RJ. . 1997 Mol. Cell. Biol. 17: 4730–4737.

  • Vyakarnam A, Lenneman AJ, Lakkides KM, Patterson RJ and Wang JL. . 1998 Exp. Cell. Res. 242: 419–428.

  • Wada J and Kanwar YS. . 1997 J. Biol. Chem. 272: 6078–6086.

  • Wagner-Hulsmann C, Bachinski N, Diehl-Seifert B, Blumbach B, Steffen R, Pancer Z and Muller WE. . 1996 Glycobiology 6: 785–793.

  • Walter MA, Spillett DJ, Thomas P, Weissenbach J and Goodfellow PN. . 1994 Nat. Genet. 7: 22–28.

  • Welch DR, Chen P, Miele ME, McGary CT, Bower JM, Stanbridge EJ and Weissman BE. . 1994 Oncogene 9: 255–262.

  • Willis AE. . 1999 Int. J. Biochem. Cell. Biol. 31: 73–86.

  • Yang RY, Hsu DK and Liu FT. . 1996 Proc. Natl. Acad. Sci. USA 93: 6737–6742.

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Acknowledgements

The present research was funded in part by National Institutes of Health Grant CA74468, an award from the Samuel Waxman Cancer Research Foundation and the Chernow Endowment. PB Fisher is the Michael and Stella Chernow Research Scientist. We gratefully acknowledge K Huebner and T Druck for help in mapping of the chromosomal location of PCTA-1.

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  1. Terry Roberts

    Present address: Imperial Cancer Research Foundation, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK

Authors and Affiliations

  1. Department of Urology, Herbert Irving Comprehensive Cancer Center, Columbia University, College of Physicians and Surgeons, New York, 10032, NY, USA

    Rahul V Gopalkrishnan, Terry Roberts, Sandeep Tuli, Dong-chul Kang, Keith A Christiansen & Paul B Fisher

  2. Department of Neurosurgery, Herbert Irving Comprehensive Cancer Center, Columbia University, College of Physicians and Surgeons, New York, 10032, NY, USA

    Paul B Fisher

  3. Department of Pathology, Herbert Irving Comprehensive Cancer Center, Columbia University, College of Physicians and Surgeons, New York, 10032, NY, USA

    Paul B Fisher

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Gopalkrishnan, R., Roberts, T., Tuli, S. et al. Molecular characterization of prostate carcinoma tumor antigen-1, PCTA-1, a human Galectin-8 related gene. Oncogene 19, 4405–4416 (2000). https://doi.org/10.1038/sj.onc.1203767

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