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v-Myb can transform and regulate the differentiation of melanocyte precursors

Oncogene volume 18, pages 7226–7233 (1999)Cite this article

Abstract

The activity of the c-Myb transcription factor is essential for the development of definitive multi- and uni-lineage progenitors of the haemopoietic system. Reflecting this requirement, c-Myb has been oncogenically activated by transduction in the E26 avian retrovirus which elicits an acute leukaemia by transforming haemopoietic progenitors. Here, we report the novel finding that Myb in cooperation with EGF receptor signalling can be used to generate clonally expanded populations of transformed cells which have the phenotype of melanocyte precursors. Through the use of a conditional temperature sensitive mutant of Myb, we show that in the transformed cells Myb regulates commitment to melanocyte differentiation and possibly proliferation. These results add to our understanding of the roles of c-Myb beyond the haemopoietic system and to our knowledge and means of investigating the importance of transcription factors in the melanocyte linage.

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Acknowledgements

We thank Anthony Crawford for technical assistance. We are especially grateful to Nicole Le Douarin, Catherine Ziller, Professor Horton and Oli Vainio for the gifts of antibodies, and to Makato Mochii for the MMP115 cDNA. This work was supported by the Wellcome Trust and the Royal Society. J Frampton is the recipient of a Wellcome Trust Senior Research Fellowship in Basic Biomedical Science.

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  1. Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, UK

    Martyn V Bell & Jonathan Frampton

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Bell, M., Frampton, J. v-Myb can transform and regulate the differentiation of melanocyte precursors. Oncogene 18, 7226–7233 (1999). https://doi.org/10.1038/sj.onc.1203161

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