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Refinement of the LOH region 1 at 11q23.1 deleted in human breast carcinomas and sublocalization of 11 expressed sequence tags within the refined region

Oncogene volume 18, pages 1635–1638 (1999)Cite this article

Abstract

Loss of constitutive heterozygosity at 11q23 has been detected in various human solid tumors. Here, we described the analysis of a series of normal and tumor pairs from 110 breast carcinomas for the presence of loss of heterozygosity at 11q23 loci. The overall frequency of LOH was 48%, confirming the importance of deletions at 11q23 in breast tumorigenesis. Previously, we have identified two independent regions of LOH at 11q23, the LOH region 1 at 11q23.1 and the LOH region 2 at 11q23.3. The most telomeric region was recently refined between loci D11S1345 and D11S1316, a region of about 1 Mb. However, the LOH region 1, most centromeric, was still not finely refined: the boundaries were defined by loci D11S2000 and D11S897, separated by about 8 Mb. Here, we refined its boundaries between loci D11S1347 and D11S927, a region of about 2 Mb. We have mapped 11 expressed sequence tags (ESTs) within this region and excluded another 20. This study represents a further step toward the identification of the putative tumor suppressor gene found within the LOH region 1 at 11q23.1.

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Acknowledgements

We are grateful to Augusto Bevilacqua and Pietro Zucchini for technical assistance. This work was supported by grants from the EC BIOMED contract BMH4-CT95-0914 and from the Associazione Italiana per la Ricerca sul Cancro (AIRC). GC was supported by a IARC Research Training Fellowship. MGdI was supported by a fellowship from the Fondazione Italiana per la Ricerca Sul Cancro.

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Authors and Affiliations

  1. Department of Molecular and Diagnostic Medicine, University of Ferrara, via Luigi Borsari, 46, Ferrara, 44100, Italy

    Maria Grazia di Iasio, George Calin, Giuseppe Barbanti-Brodano & Massimo Negrini

  2. Department of Clinical e Biological Science, University of Pavia, Varese, I-21100, Italy

    Maria Grazia Tibiletti

  3. Department of Bioscience at Novum, Karolinska Institute, S-14157 Huddinge, Sweden

    Igor Vorechovsky

  4. Department of Surgery, Huddinge Hospital, S-14157 Huddinge, Sweden

    Kristinn P Benediktsson

  5. Department of Structural and Functional Biology, University of Varese, I-21110 Varese, Italy

    Roberto Taramelli

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  1. Maria Grazia di Iasio
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di Iasio, M., Calin, G., Tibiletti, M. et al. Refinement of the LOH region 1 at 11q23.1 deleted in human breast carcinomas and sublocalization of 11 expressed sequence tags within the refined region. Oncogene 18, 1635–1638 (1999). https://doi.org/10.1038/sj.onc.1202453

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