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Growth stimulation of murine fibroblasts by TGF-β1 depends on the expression of a functional p53 protein

Oncogene volume 18, pages 703–711 (1999)Cite this article

Abstract

Transforming Growth Factor-β1 (TGF-β1) inhibits the proliferation of most cells, but stimulates some mesenchymal cell types, including murine NIH3T3 fibroblasts. We show here that TGF-β1 growth stimulation of NIH3T3 fibroblasts is reversed when these cells are transformed by SV40 or are transfected with a plasmid encoding the SV40 Large T antigen. Inversion of the TGF-β1 growth stimulation of NIH3T3 cells is not observed when these cells are transfected with plasmids expressing either a mutant Large T, unable to bind P53, or the E1A adenovirus oncoprotein which binds the retinoblastoma protein pRB but not P53. But when the TGF-β1-growth stimulated cells are transfected with a plasmid expressing a mutant form of Large T capable of binding to P53, but not to pRB, or with one expressing the E1B-55 kD adenovirus oncoprotein, which also binds to P53 but not to pRB, the cells are growth-inhibited by TGF-β1. The cdk inhibitor p21Waf is decreased in TGF-β1-stimulated NIH3T3 fibroblasts and increased in TGF-β1-inhibited SV40-transformed cells. Finally, we show that T12 fibroblasts, from a P53 knockout mouse, are growth inhibited by TGF-β1 and that they remain so upon transfection with a P53 which is mutant at restrictive temperature, but become growth-stimulated by this factor at permissive temperature when P53 is functional. These data strongly suggest that growth-stimulation of fibroblasts by TGF-β1 depends on the presence of a functional P53 protein and that inversion of this response occurs if P53 is absent or inactivated.

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Acknowledgements

We thank Drs E and P May, A Zantema, AJ van der Eb and D Trouche for supplying certain reagents and Zohar Mischal for the flow cytometry analysis. This work received financial suport from the `Association de Recherche sur le Cancer' and the Fondation de France. F.D. was financed by the `Association de Recherche sur le Cancer' (96-97) and by the `Ligue Nationale Contre le Cancer, Comité de l'Essonne' (97-98). SR received support from the `Fondation Singer-Polignac'. This work was started whilst all the authors were still at the Institut Curie, Orsay.

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  1. David A Lawrence: Author for correspondence

Authors and Affiliations

  1. UPR9079 du CNRS, BP n°8, Villejuif, 94801

    Fatima Dkhissi, Stéphane Raynal & David A Lawrence

  2. UMR146 du CNRS, Institut Curie, 91405, Orsay, France

    Pierre Jullien

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  1. Fatima Dkhissi
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Dkhissi, F., Raynal, S., Jullien, P. et al. Growth stimulation of murine fibroblasts by TGF-β1 depends on the expression of a functional p53 protein. Oncogene 18, 703–711 (1999). https://doi.org/10.1038/sj.onc.1202341

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