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Differential gene expression in neoplastic and human papillomavirus-immortalized oral keratinocytes

Oncogene volume 18, pages 827–831 (1999)Cite this article

Abstract

We have previously demonstrated that normal human oral keratinocytes immortalized by transfection with human papillomavirus type-16 Dna became tumorigenic after exposure to a chemical carcinogen. In an effort to detect differentially regulated genes associated with this transition from the immortal to the malignant phenotype, we employed representational differences analysis (a PCR-coupled subtractive hybridization technique). After analysing 50 colonies, 12 putative messages were identified. Northern analysis comparison using the identified cDNAs as probes was made between normal human oral keratinocyte, papillomavirus-immortalized human oral keratinocytes (HOK-16B), a neoplastic cell line derived from HOK-16B (HOK-16B-BaP-T) and the human oral cancer cell lines Hep-2, SCC-9 and Tu-177. We found that mRNAs encoding for cyclophilin A, c-myc binding protein 1, the heat shock protein 90α and one unknown transcript were up-regulated in the oral cancer cell lines analysed as well as in HOK-16B cells. We also detected a downregulation of the mRNAs encoding the skin-derived antileukoproteinase SKALP/elafin, the translationally regulated p23 protein and one unknown transcript. Whether these messages are associated to the neoplastic conversion of human keratinocytes remains to be determined.

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Acknowledgements

We would like to thank Bradley Ackerson and Wentong Guo for critical reading of this manuscript. This work was, in part, supported by grants DE11728 and DE10598 from the National Institute of Dental Research.

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  1. UCLA School of Dentistry and Dental Research Institute, Los Angeles, 90095-1668, California, USA

    Osvaldo Rey, Marcel A Baluda & No-Hee Park

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  1. Osvaldo Rey
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  2. Marcel A Baluda
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Rey, O., Baluda, M. & Park, NH. Differential gene expression in neoplastic and human papillomavirus-immortalized oral keratinocytes. Oncogene 18, 827–831 (1999). https://doi.org/10.1038/sj.onc.1202328

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