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Effect of oral oleoyl-estrone on adipose tissue composition in male rats

International Journal of Obesity volume 26, pages 1092–1102 (2002)Cite this article

Abstract

OBJECTIVE: To determine whether the oral administration of oleoyl-estrone has similar mass-decreasing effects on the main different sites of white adipose tissue (WAT).

DESIGN: Adult male Zucker lean rats were given a daily oral gavage of oleoyl-estrone (OE, 10 µmol/kg) in 0.2 ml of sunflower oil for 10 days, and were compared with controls receiving only the oil. The mass of the main WAT sites: subcutaneous, epididymal, mesenteric, retroperitoneal, gluteal, perirenal and interscapular, as well as perirenal and interscapular brown adipose tissue (BAT), were dissected and studied.

MEASUREMENTS: The tissue weight, DNA, protein, lipid and total cholesterol content, together with the levels of leptin and acyl-estrone in the larger WAT and BAT masses, were measured.

RESULTS: The weights of WAT depots were correlated with body weight but those of BAT were not. Cell size was maximal for epididymal and mesenteric and minimal for subcutaneous and retroperitoneal WAT and BAT. Differences were detected in DNA, and in protein and lipid content between distinct WAT sites. OE treatment tended to decrease cell number and cell size in WAT; only small differences in composition were found between WAT locations inside the visceral cavity and those outside. Decreases in lipid content were maximal in mesenteric fat. Leptin and acyl-estrone content were fairly uniform at the different WAT sites, except for high concentrations in gluteal WAT. OE induced a greater decrease in leptin and acyl-estrone than in DNA and lipids; changes in these hormones were fairly parallel in all sites.

CONCLUSIONS: In general, the differences in composition between visceral and peripheral subcutaneous WAT and their responses to OE were less marked than the individual differences observed between specific sites, regardless of location. WAT sites are fairly diverse in composition, but their response to OE treatment was uniform. OE decreased the weight of WAT through reduction of both cell numbers and size; but did not change the mass or composition of BAT significantly. The effects of OE are more marked in the hormonal signals (leptin and acyl-estrone) from the tissue than in its composition and mass.

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Acknowledgements

This study was financed by grants 2FD97-0233 and AGL2000-0780 of the Government of Spain. We thank the Language Advisory Service at the University of Barcelona for correction of the text.

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Authors and Affiliations

  1. Centre Especial de Recerca en Nutrició i Ciència dels Aliments, Universitat de Barcelona, Barcelona, Spain

    X Remesar, JA Fernández-López, MT Blay, P Savall, A Salas, M Díaz-Silva, M Esteve, MM Grasa & M Alemany

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Correspondence to M Alemany.

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Remesar, X., Fernández-López, J., Blay, M. et al. Effect of oral oleoyl-estrone on adipose tissue composition in male rats. Int J Obes 26, 1092–1102 (2002). https://doi.org/10.1038/sj.ijo.0802056

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