Abstract
Pro-opiomelanocortin (POMC)-derived peptides play a critical role in body weight regulation in the central nervous system. Mice deficient in POMC developed obesity. We sought mutations in the POMC gene in 50 morbidly obese (body mass index 35–60 kg/m2) Japanese subjects with diabetes by direct sequencing. Apart from two silent mutations (C6982T and C7285T), no other mutations were detected. Frequencies of these mutations were not significantly different between 100 obese subjects and 100 controls. Also, the frequencies did not differ in the subjects with or without diabetes. These results suggest that mutations in the POMC gene are unlikely to be a major factor of obesity or diabetes in Japanese subjects.
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References
Barsh GS, Farooqi IS, O'Rahilly S . Genetics of body-weight regulation Nature 2000 404: 644–651.
Huszar D, Lynch CA, Fairchild-Huntress V, Dunmore JH, Fang Q, Berkemeier LR, Gu W, Kesterson RA, Boston BA, Cone RD, Smith FJ, Campfield LA, Burn P, Lee F . Targeted disruption of the melanocortin-4 receptor results in obesity in mice Cell 1997 88: 131–141.
Krude H, Biebermann H, Luck W, Horn R, Brabant G, Gruters A . Severe early-onset obesity, adrenal insufficiency and red hair pigmentation caused by POMC mutations in humans Nature Genet 1998 19: 155–157.
Yeo GS, Farooqi IS, Aminian S, Halsall DJ, Stanhope RG, O'Rahilly S . A frameshift mutation in MC4R associated with dominantly inherited human obesity Nature Genet 1998 20: 111–112.
Vaisse C, Clement K, Guy-Grand B, Froguel P . A frameshift mutation in human MC4R is associated with a dominant form of obesity Nature Genet 1998 20: 113–114.
Bray GA, Tartaglia LA . Medicinal strategies in the treatment of obesity Nature 2000 404: 672–677.
Ohshiro Y, Sanke T, Ueda K, Shimajiri Y, Nakagawa T, Tsunoda K, Nishi M, Sasaki H, Takasu N, Nanjo K . Molecular scanning for mutations in the melanocortin-4 receptor gene in obese/diabetic Japanese Ann Hum Genet 1999 63: 483–487.
Ohshiro Y, Ueda K, Nishi M, Shigame M, Wakasaki H, Kawashima H, Furuta H, Sasaki H, Sanke T, Takasu N, Nanjo K . A polymorphic marker in the leptin gene associated with Japanese morbid obesity J Mol Med 2000 78: 516–520.
Hinney A, Becker I, Heibult O, Nottebom K, Schmidt A, Ziegler A, Mayer H, Siegfried W, Blum WF, Remschmidt H, Hebebrand J . Systematic mutation screening of the pro-opiomelanocortin gene: identification of several genetic variants including three different insertions, one nonsense and two missense point mutations in probands of different weight extremes J Clin Endocrinol Metab 1998 83: 3737–3741.
Takahashi H, Hakamata Y, Watanabe Y, Kikuno R, Miyata T, Numa H . Complete nucleotide sequence of the human corticotropin-β-lipotropin precursor gene Nucleic Acids Res 1983 11: 6847–6858.
Acknowledgements
This study was supported in part by Health Science research grants (Research on Human Genome Therapy) from the Ministry of Health Welfare and a grant from the Japanese Ministry of Education (12204092).
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Ohshiro, Y., Ueda, K., Wakasaki, H. et al. Sequence analysis of the pro-opiomelanocortin (POMC) gene in obese/diabetic Japanese. Int J Obes 26, 730–731 (2002). https://doi.org/10.1038/sj.ijo.0801950
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DOI: https://doi.org/10.1038/sj.ijo.0801950
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