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UCP2 muscle gene transfer modifies mitochondrial membrane potential

International Journal of Obesity volume 25, pages 68–74 (2001)Cite this article

Abstract

OBJECTIVE: The aim of this work was to evaluate the effect of uncoupling protein 2 (UCP2) muscle gene transfer on mitochondrial activity.

DESIGN: Five week-old male Wistar rats received an intramuscular injection of plasmid pXU1 containing UCP2 cDNA in the right tibialis anterior muscles. Left tibialis anterior muscles were injected with vehicle as control. Ten days after DNA injection, tibialis anterior muscles were dissected and muscle mitochondria isolated and analyzed.

RESULTS: There were two mitochondrial populations in the muscle after UCP2 gene transfer, one of low fluorescence and complexity and the other, showing high fluorescence and complexity. UCP2 gene transfer resulted in a 3.6 fold increase in muscle UCP2 protein levels compared to control muscles assessed by Western blotting. Furthermore, a significant reduction in mitochondria membrane potential assessed by spectrofluorometry and flow cytometry was observed. The mitochondria membrane potential reduction might account for a decrease in fluorescence of the low fluorescence mitochondrial subpopulation.

CONCLUSION: It has been demonstrated that UCP2 muscle gene transfer in vivo is associated with a lower mitochondria membrane potential. Our results suggest the potential involvement of UCP2 in uncoupling respiration.

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Acknowledgements

The rat UCP2 cDNA was kindly provided by Dr Warden and Dr Ricquier. This work was partially supported by a grant from the University of Navarra, L.E.98 (Nutrition and Obesity) and Gobierno de Navarra (project no 1/98 de OF 32/98). The technical assistance of V Ciaúrriz and A Lorente is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Physiology and Nutrition, University of Navarra, Pamplona, Spain

    A Marti, E Larrarte, M Garcia & JA Martinez

  2. Department of Genetics, University of Navarra, Pamplona, Spain

    FJ Novo

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  1. A Marti
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  2. E Larrarte
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  3. FJ Novo
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Correspondence to JA Martinez.

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Marti, A., Larrarte, E., Novo, F. et al. UCP2 muscle gene transfer modifies mitochondrial membrane potential. Int J Obes 25, 68–74 (2001). https://doi.org/10.1038/sj.ijo.0801484

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