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In vivo effects of CGP-12177 on the expression of leptin and uncoupling protein genes in mouse brown and white adipose tissues

International Journal of Obesity volume 24, pages 423–428 (2000)Cite this article

Abstract

OBJECTIVE: To assess the effect of chronic treatment with CGP-12177 a β3-adrenergic receptor (AR) agonist with β2/β1-AR antagonist action, on the expression of the leptin gene and of genes coding for uncoupling proteins (ucp1, ucp2 and ucp3) in brown and white adipose tissues.

DESIGN: NMRI mice received a daily subcutaneous injection of CGP-12177 at a dose of 0.05, 0.2, 0.5 or 1 mg/kg for 15 days. The specific levels of the mRNAs of interest were analysed in interscapular brown adipose tissue (BAT) and in two white adipose tissue (WAT) depots, inguinal (IWAT) and epididymal (EWAT).

RESULTS: No changes in food intake or body weight were detected at any dose of CGP-12177. In the two WAT depots, the treatment led to enhanced expression of ucp1 and ucp3, but not of ucp2. In BAT, low doses (0.05 and 0.2 mg/kg) led to a decreased expression of the three ucp genes, whereas a slight stimulatory effect on the three ucp genes was elicited with a high dose (1 mg/kg). Treated animals displayed increased expression of leptin in BAT and, to a lesser extent, in IWAT, but not in EWAT.

CONCLUSION: The results reveal that simultaneous stimulation of the expression of certain ucp genes and the leptin gene can be achieved, and suggest that adrenergic regulation of the leptin gene and of genes of the ucp family in adipose tissues is the result of complex interactions between the different β-AR pathways.

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Acknowledgements

This work was funded by Dirección General de Enseñanza Superior e Investigación Científica, Ministerio de Educación y Ciencia, Spain (grant PM97-0094 to A.P.) and by the European Commission DGXII (contract no. ERBCHRX©CT94O49O and COST 918).

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  1. Departament de Biologia Fonamental i Ciències de la Salut, Laboratori de Biologia Molecular, Nutrició i Biotecnologia, Universitat de les Illes Balears, Palma de Mallorca, Spain

    P Oliver, C Picó, N Martínez, M L Bonet & A Palou

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  1. P Oliver
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  3. N Martínez
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  4. M L Bonet
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Correspondence to A Palou.

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Oliver, P., Picó, C., Martínez, N. et al. In vivo effects of CGP-12177 on the expression of leptin and uncoupling protein genes in mouse brown and white adipose tissues. Int J Obes 24, 423–428 (2000). https://doi.org/10.1038/sj.ijo.0801174

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