Abstract
OBJECTIVE: To search the human ob gene for mutations and evaluate their role in massive obesity.
DESIGN: Direct mutation screening of the gene and case-control association study. Multivariate analyses for evaluation of differences in clinical parameters.
SUBJECTS: Primary mutation screening: 24 morbidly obese subjects (body mass index (BMI) >40 kg/m2). Association study: 395 unrelated morbidly obese subjects (BMI >40 kg/m2), 121 lean, non-diabetic control individuals, 72 women of a random sample with an average BMI 32.5 kg/m2.
RESULTS: We report the finding of a DNA variant in exon 1 of the human ob gene (A−>G substitution, base +19). This variant showed a prevalence of 62% in our study population. Association analyses under different genetic models (dominant, co-dominant, recessive) showed no significant evidence for an association of this variant with BMI. However, obese individuals homozygous for the G-allele showed significantly lower leptin concentrations compared to obese patients either heterozygous or homozygous for the A-allele after correction for BMI.
CONCLUSION: Recent linkage studies have shown evidence for linkage of the hsob locus with obesity. Our study provides further evidence that a defect in the ob gene in linkage disequilibrium with the G-allele of exon 1 might be involved in obesity by affecting leptin concentrations.
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Hager, J., Clement, K., Francke, S. et al. A polymorphism in the 5′ untranslated region of the human ob gene is associated with low leptin levels. Int J Obes 22, 200–205 (1998). https://doi.org/10.1038/sj.ijo.0800567
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DOI: https://doi.org/10.1038/sj.ijo.0800567
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