Abstract
Aim:
The aim of the present study was to investigate the effectiveness of transforming growth factor (TGF)-β1 antisense oligodeoxynucleotides (ODN) in ameliorating deteriorated kidney function in rats with puromycin-induced chronic renal failure (CRF).
Methods:
Saline, puromycin, puromycin+TGF-β1 antisense ODN or puromycin+scrambled ODN were administered to unilaterally nephrectomized rats. Renal hemodynamic and excretory measurements were taken in the anaesthetized rats that had undergone surgical procedure.
Results:
It was observed that in the CRF rats, there was a marked reduction in the renal blood flow (RBF), glomerular filtration rate (GFR), severe proteinuria, and almost 6-fold increased fractional excretion of sodium (FE Na+) as compared to that in the control rats (all P<0.05). It was further observed that in the CRF rats, the treatment with TGF-β1 antisense, but not scrambled ODN, markedly attenuated the reduction of RBF, GFR, and proteinuria and markedly prevented the increase of the FE Na+ (all P<0.05). In addition, the renal hypertrophy in the CRF group (P<0.05 vs non-renal failure control) was markedly attenuated after treatment with TGF-1 antisense ODN (P<0.05). Focal segmental glomerulosclerosis was evident only in the untreated and scrambled ODN-treated CRF groups. An interesting observation of this study was that in the CRF rats, although there was marked attenuating and preventive effects of the TGF-β1 antisense ODN on the deteriorated renal functions, the antisense treatment did not cause any marked change in the renal expression of TGF-β1 at the protein level.
Conclusion:
Collectively, the data obtained suggests that TGF-β1 antisense ODN possesses beneficial effects in puromycin-induced chronic renal failure and that the deterioration in morphology and impaired renal function in this pathological state is in part dependent upon the action of TGF-β1 within the kidney.
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This research was funded by a grant from the Malaysian Government (IRPA grant No 06-02-03-0541).
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Hiong, L., Voon, K., Abdullah, N. et al. Effect of TGF-β1 antisense oligodeoxynucleotide on renal function in chronic renal failure rats. Acta Pharmacol Sin 29, 451–457 (2008). https://doi.org/10.1111/j.1745-7254.2008.00772.x
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DOI: https://doi.org/10.1111/j.1745-7254.2008.00772.x
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