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To fully understand human health and allow everyone access to advances in biomedicine, research programmes must include participants from diverse backgrounds. Yet, a large proportion of participants in current biobanks are of European ancestries. To address this disparity, the All of Us research programme aims to collect health and genomic data of at least 1 million participants, who represent the racial, ethnic and ancestral diversity across the United States. This Collection presents primary research articles and related content that describe and utilize the genomic data from the All of Us research programme.