Abstract
Sialic acid (SA) is present at the terminal ends of carbohydrate chains in glycoproteins and glycolipids and is involved in various biological phenomena. The biological function of the disialyl-T (SAα2-3Galβ1-3(SAα2-6)GalNAcα1-O-Ser/Thr) structure is largely unknown. To elucidate the role of disialyl-T structure and determine the key enzyme from the N-acetylgalactosaminide α2,6-sialyltransferase (St6galnac) family involved in its in vivo synthesis, we generated St6galnac3- and St6galnac4-deficient mice. Both single-knockout mice developed normally without any prominent phenotypic abnormalities. However, the St6galnac3::St6galnact4 double knockout (DKO) mice showed spontaneous hemorrhage of the lymph nodes (LN). To identify the cause of bleeding in the LN, we examined podoplanin, which modifies the disialyl-T structures. The protein expression of podoplanin in the LN of DKO mice was similar to that in wild-type mice. However, the reactivity of MALII lectin, which recognizes disialyl-T, in podoplanin immunoprecipitated from DKO LN was completely abolished. Moreover, the expression of vascular endothelial cadherin was reduced on the cell surface of high endothelial venule (HEV) in the LN, suggesting that hemorrhage was caused by the structural disruption of HEV. These results suggest that podoplanin possesses disialyl-T structure in mice LN and that both St6galnac3 and St6galnac4 are required for disialyl-T synthesis.
Similar content being viewed by others
Introduction
Sialylation is the process of adding sialic acid to the non-reducing ends of various glycoconjugates. Sialic acid is a negatively charged monosaccharide added to many types of glycans, such as N-glycans and O-glycans (O-GalNAc (mucin-type), O-Mannose, O-Fucose, and O-GlcNAc), proteins, and glycolipids, and is involved in many important biological processes such as cell adhesion, signal transduction, and immune regulation1,2,3. Sialylation is tightly regulated by sialyltransferases localized in the Golgi membrane. Twenty different sialyltransferases attach sialic acids to glycans via α2-3, α2-6, and α2-8 linkages. Among them, sialic acid is attached to GalNAc by α2,6-linkages only in mucin-type O-glycans and glycolipids. The sialyl-Tn (STn: Sialic acid α2-6GalNAc-Ser/Thr) and disialyl-T (Sialic acid α2-3Galβ1-3(sialic acid α2-6)GalNAc-Ser/Thr) antigens on mucin-type glycans are well-known cancer-associated glycans4,5; however, their physiological functions are largely unknown. According to in vitro substrate specificity analysis, the biosynthesis of these antigens is catalyzed by four types of N-acetylgalactosaminide α- 2–6-sialyltransferases (St6galnac1, St6galnac2, St6galnac3, and St6galnac4), which attach sialic acid to GalNAc via α2,6-linkages5,6 (Fig. 1A). Each pair of St6galnac1 and St6galnac2 or St6galnac3 and St6galnac4 genes has a high gene sequence homology. In vitro enzymatic analysis has shown that St6galnac1 and St6galnac2 have the highest specific activity for synthesis of the STn antigen, while St6galnac3 and St6galnac4 have the highest specific activity for synthesis of the disialyl-T antigen. STn is almost absent in normal tissues, while disialyl-T is found in normal tissues; however, the roles of St6galnac3 and St6galnac4 and the subsequent disialyl-T antigen synthesized by these enzymes are still unclear.
Podoplanin is an O-linked glycosylated type I membrane protein with some highly conserved Platelet Aggregation-stimulating (PLAG) domains. Podoplanin is modified with core 1-derived sialylated O-glycans7,8,9. Furthermore, disialyl-T, a type of core 1-derived O-glycan, is modified in at least one of its PLAG domains. Podoplanin is expressed in the heart, type 1 alveolar epithelium, renal glomerular podocytes, epithelial cells of the brain choroid plexus, glial cells, lymphatic endothelial cells, fibroblastic reticular cells, and stromal cells in lymph nodes. Podoplanin-knockout (KO) mice exhibit prenatal and postnatal lethality, including cardiac defects, abnormal lung development, lymphedema, hemorrhage of lymphatic vessels, and the absence of the lymph nodes10,11,12,13,14. These observations indicate that podoplanin plays important roles in the developmental processes. It is also upregulated in brain tumors, lung cancer, and cervical cancer and is known to be involved in cancer invasion and prognosis15,16.
C-type lectin like-receptor 2 (Clec-2) is a functional protein that recognizes podoplanin in vivo. Clec-2 is highly expressed, particularly in platelets and megakaryocytes. The binding of podoplanin to Clec-2 causes platelet activation17,18,19,20,21 and plays vital roles in the separation of blood and lymphatic vessels during in vivo development; in maintaining the integrity of high endothelial venule (HEV) structure, a specialized vascular structure in lymph nodes22; and in antigen presentation by the dendritic cells. Clec-2 binds through the disialyl-T structure and podoplanin peptide7,8. However, the significance of the disialyl-T structure is still unclear, as previous studies have only analyzed podoplanin-KO and Clec-2-KO cells and mice. Podoplanin may be a promising target protein for developing anti-metastatic drugs, as the Clec-2/podoplanin interaction causes platelet aggregation in the bloodstream, thereby promoting hematogenous tumor metastasis23,24. To leverage their interaction for therapy, it is essential to clarify the role of the disialyl-T structure in normal tissue conditions. Therefore, we aimed to elucidate the effect of the disialyl-T structure on podoplanin, determine other additional roles of the disialyl-T structure, and identify the enzyme that synthesizes disialyl-T in vivo. Furthermore, to clarify the role of the disialyl-T structure and determine the enzymes catalyzing the synthesis of this antigen in vivo, we generated double KO mice deficient for St6galnac3 and St6galnac4 and for St6galnac3::St6galnac4 and conducted their phenotypic analysis.
Results
Generation of St6galnac3 KO, St6galnac4 KO, and St6galnac3::4 DKO mice
In vitro substrate specificity and enzymatic activity analysis in previous studies have demonstrated that four of the six isozymes of the St6galnac family, namely St6galnac1, 2, 3, and 4, are necessary for synthesis of the disialyl-T (SAα2-3Galβ1-3 (SAα2-6) GalNAcα1-O-Ser/Thr) structure on mucin-type O-glycans (Fig. 1A)25. To elucidate the biological functions of the disialyl-T antigen, we mainly focused on St6galnac3 and St6galnac4, which have specific in vitro enzymatic properties, and generated their KO mice using the CRISPR/Cas9 system. Four pX330 vectors containing the target sequences to exclude exon 3 of St6galnac3 and exons 2–5 of St6galnac4 were simultaneously microinjected into the pronuclei of fertilized oocytes obtained from C57BL/6 J mice (Fig. 1B). We generated St6galnac3 KO (3KO), St6galnac4 KO (4KO), and St6galnac3::St6galnac4 double KO (DKO) mice from a single parent mouse, and the genetically engineered mice were distinguishable upon PCR amplification of the genomic DNA isolated from their tails (Fig. 1C). Mating between St6galnac3+/- mice or between St6galnac4+/− mice produced offspring with normal Mendelian inheritance (Tables S1 and S2). The DKO mice were generated by crossing St6galnac3+/−::St6galnac4+/− mice with each other.
Disialyl-T structures are synthesized by St6galnac3 and St6galnac4 in vivo
The 3KO, 4KO, and DKO mice grew to adulthood with no apparent problems and lived for approximately 2 years, similar to the wild-type (WT) mice. There were no changes in the body weights (Fig. S1A) and the number of white blood cells, red blood cells, and platelets (Fig. S1B–D). The weights of major organs of mutant male mice from the four groups were similar to those of the WT mice (Fig. S1E,F).
Lectins are classically used to estimate glycan structures and have been purified from animals and plants; Maackia Amurensis Lectin II (MALII, also known as Maackia amurensis hemagglutinin; MAH) was reported as a leguminous lectin that recognizes disialyl-T26. To confirm changes in the structures of glycans in several tissues, we performed immunofluorescence staining with MALII lectin. MALII reactivity was reduced in the lung, stomach, and spleen in DKO, with no histological changes observed (Fig. 1D). In other tissues, such as the cerebral cortex, cerebellum, heart, duodenum, testis, epididymis, kidney, and epididymal white adipose tissue, hematoxylin and eosin (HE) and MALII staining results were similar in the WT and DKO mice (Fig. S2). Moreover, to compare the amounts of disialyl-T structures in several tissues, we performed lectin blotting using MALII lectin. The results showed a remarkable decrease in the expression of disialyl-T in several tissues, including the brain, heart, lung, liver, kidney, bladder, skeletal muscle, spleen, stomach, duodenum, colon, testis, epididymis, and thymus, in entire lane profile (Fig. 1E).
To examine whether St6galnac3 and St6galnac4 can modify α2,6-sialic acids which constitute disialyl-T, we evaluated the reactivity of MALII and Jacalin by dot blot analysis using brain tissues of WT and DKO mice because expression of the disialyl-T structure has been confirmed in the brain by mass spectrometry27,28. Jacalin, a lectin isolated from jackfruit, has binding specificity mainly for galactose-β1,3-N-acetylgalactosamine, which constitutes disialyl-T. Hence, Jacalin can be used to evaluate disialyl-T structure as well as the reactivity of MALII, as it does not prefer binding of α2,6-sialylation, while α2,3-sialylation is tolerated29,30. Results showed that the reactivity of MALII lectin was reduced in DKO mice compared to that in WT mice, and the difference was eliminated by treatment with α2,3-,6-,8-,9-sialidase from Arthrobacter ureafaciens (Fig. 1F). Contrary to these results, the reactivity of Jacalin was increased in DKO mice compared to that in WT mice. Furthermore, the difference in reactivity of Jacalin was also eliminated by sialidase treatment (Fig. 1G). These results suggest reduced reactivity of MALII in DKO mice is due to the loss of α 2,6-sialylation of disialyl-T structure.
In addition, MALII blotting was performed using the spleens of 3KO and 4KO mice. The results indicated that the reduction in MALII reactivity was more significant in DKO mice than in WT, 3KO, and 4KO mice, especially with regard to a band around 45 kDa (Fig. 1H).
To confirm the key enzymes involved in the synthesis of disialyl-T structures in vivo, we evaluated the tissue distribution of the expression of four St6galnac genes in 8-week-old male C57BL/6 J mice using quantitative real-time RT-PCR (Fig. S3). Plasmids with known DNA concentrations were used to plot a standard curve to perform a comparative expression analysis of different St6galnac genes. The expression of St6galnac2 and St6galnac4 was more dominant than that of St6galnac1 and St6galnac3 overall. Of the four enzymes, St6galnac2 showed the highest expression, predominantly in the gastrointestinal and reproductive tissues than in the brain and tissues of immune system including the thymus, spleen, and bone marrow. In contrast, St6galnac4 showed a higher expression in the brain and immune system than in the gastrointestinal system. In addition, the tissue distribution of St6galnac3 and St6galnac4 was similar, and St6galnac3 and St6galnac4 were expressed in a wide range of tissues. Lectin blotting (Fig. 1E) and immunofluorescence staining (Figs. 1D, Fig. S2) of MALII lectins showed a reduction in their reactivity in DKO mice compared to WT mice, but did not disappear completely, suggesting that other St6galnac isozymes, such as St6galnac1 and St6galnac2, may be involved in the synthesis of the disialyl-T structure in vivo.
St6galnac3 and St6galnac4 synthesize the disialyl-T structures expressed in T cells
Because we observed a reduction in the reactivity of MALII in lymphocytes and the spleen of DKO mice (Fig. 1D), the number of T cells in the thymus and T cells/B cells in the spleen was confirmed using immunofluorescence staining and flow cytometry analysis. We observed a decreased reactivity of MALII in CD3-positive T cells of the thymus and spleens of DKO mice (Fig. S4A,B). In contrast, co-localization with MALII was not observed in the B220-positive B cells of the spleens of WT and DKO mice (Fig. S4B). However, flow cytometry analysis of the thymus and spleen showed no significant changes in the populations of major immune cells among all the mutant mice as compared to those in WT mice (Fig. S4C,D).
St6galnac3::St6galnac4 DKO mice exhibit bleeding in the lymph nodes
In DKO mice, the peripheral lymph nodes, including submandibular, axillary, inguinal, and mesenteric lymph nodes, showed increased bleeding (Fig. 2A). Particularly, hemorrhages were observed in more than 80% of DKO mice in the submandibular, axillary, and mesenteric lymph nodes. In addition, the submandibular lymph nodes of 3KO and 4KO mice also showed hemorrhages. To investigate the cause of these hemorrhages, we performed histological analysis, using HE staining, of the submandibular lymph nodes. As a result, erythrocytes were identified outside the blood vessels in the subcapsular sinus, medullary sinus, and paracortex in DKO mice (Fig. 2B). Furthermore, to efficiently generate DKO mice, 3KO:: St6galnac4+/− mice were mated with each other and 3KO mice were used as the control and we performed immunostaining for a detailed examination of erythrocyte localization in mesenteric lymph nodes, where the frequency of hemorrhage was particularly high in DKO mice, as shown in Fig. 2A. In 3KO mice, Ter119 signals, which are erythrocyte markers, were not observed in the Lyve-1-positive area that marks the lymphatic vessels (Fig. 2C). However, in DKO mice, Ter119 signals were observed in Lyve-1 and podoplanin which is a marker of lymphatic endothelial cells and fibroblastic reticular cells (Fig. 2C). These results suggest that in DKO mice, erythrocytes leak out of the blood vessels into the lymph nodes and may flow through the lymphatic vessels to the edge of lymph nodes.
During the embryonic stage, the interaction of podoplanin and Clec-2 induces the blood–lymphatic vessel separation17,19,20,31. Therefore, we determined whether the development of blood and lymphatic vessels was affected in DKO mice. However, visual inspection revealed no blood inflow into the lymphatic vessels in the mesenteries of DKO mice (Fig. 2D). In addition, blood flow into the thoracic duct was not observed in DKO mice (Fig. 2E), contrary to that in a previous report32.
Mouse podoplanin is modified with disialyl-T structures by St6galnac3 and St6galnac4 in the lymph nodes
We evaluated the gene expression of 4 St6galnac isozymes using quantitative RT-PCR to determine the key enzyme of disialyl-T synthesis in the lymph nodes. St6galnac3 and St6galnac4 transcripts levels were dominant in the submandibular, axillary, inguinal, and mesenteric lymph nodes, whereas the expression levels of St6galnac1 and St6galnac2 were remarkably low (Fig. 3A).
To confirm whether the phenotype was mediated by the disialyl-T structures modified by the St6galnac3 and St6galnac4, we examined changes in the disialyl-T structures of the mesenteric lymph node extracts.
The expression levels of podoplanin remained unaffected in the lymph node extracts from WT and all KO mice (Fig. 3B). Immunofluorescent staining with MALII lectin showed decreased reactivity of MALII in cells expected to be lymphocytes, such as T cells in the lymph nodes (Fig. 3C). However, the localization of podoplanin and MALII did not overlap. These results were supported by the co-staining of podoplanin and MALII in the kidney (Fig. S5A). Podoplanin is expressed in podocytes and Bowman's capsule epithelial cells of the kidney. MALII only reacted with the glycoproteins expressed in podocytes and not with those expressed in the Bowman's capsule epithelial cells; interestingly, it did not co-localize with podoplanin. This result could either be due to the presence of fewer disialyl-T structures on podoplanin or due to oversaturated signals of MALII reactivity from other cells. Therefore, we performed immunoprecipitation using an anti-podoplanin antibody and determined the reactivity of MALII using the enriched samples (Fig. 3D). MALII signals were detected at similar levels in WT, 3KO, and 4KO mice; however, the signal was lost in DKO mice. Furthermore, we performed immunoprecipitation using an anti-podoplanin antibody to confirm the reactivity of MALII in other tissue extracts. Podoplanin expression levels in the DKO extracts were similar to those in WT extracts from the lung, kidney, and spleen (Fig. S5B). MALII reactivity in the kidney extracts immunoprecipitated with an anti-podoplanin antibody was also lost in the DKO samples compared to that in the WT samples (Fig. S5C). These results indicate that mouse podoplanin is modified with the disialyl-T structure in the lymph nodes and that both St6galnac3 and St6galnac4 are required for its synthesis.
VE-cadherin is reduced within the HEV area in the lymph nodes of DKO mice
The interaction between podoplanin and Clec-2 plays important roles in the embryonic and adult stages. To investigate whether the hemorrhages of lymph nodes were caused by reduced Clec-2 expression in DKO mice, we performed western blotting using an anti-Clec-2 antibody in the platelet extracts (Fig. 3E). The expression of Clec-2 in all KO mice was found to be similar to that in WT mice.
In the adult stage, the interactions of podoplanin in fibroblastic reticular cells and Clec-2 in platelets contribute to the structural integrity of HEV in the lymph nodes22. Therefore, we determined VE-cadherin expression, which is important for the cell–cell adhesion of the endothelial cells in HEV. Immunofluorescence staining using anti-VE-cadherin antibody revealed a decrease in the intensity of VE-cadherin within the HEV region, which is defined as the positive area of the peripheral node addressin (PNAd) signal, in the lymph nodes of DKO mice (Fig. 3F). We performed western blotting using an anti-VE-cadherin (CD144) antibody to compare the expression levels of VE-cadherin in the lymph nodes. VE-cadherin expression remained unchanged in all KO mice compared to that in WT mice (Fig. S6). This may be because VE-cadherin is also expressed in capillary endothelial cells, which masks the reduced expression of VE-cadherin in HEV.
Next, we determined the area of VE-cadherin for each HEV region using the results shown in Fig. 3F. There was no difference in the HEV region between littermate 3KO and DKO mice (Fig. 3G); however, the area of VE-cadherin per HEV region decreased by 20% in DKO mice compared to that in 3KO mice (Fig. 3H). These results suggest that a partial decrease in VE-cadherin expression may promote the leakage of erythrocytes from HEVs.
Discussion
The role of disialyl-T structures has been largely unknown due to the lack of knowledge regarding the in vivo substrate specificity of the St6galnac family. In this study, we generated 3KO, 4KO, and DKO mice using the CRISPR/Cas9 system and performed phenotypic analysis. MALII lectin analysis revealed that the disialyl-T structure is partially reduced in some tissues of DKO mice. Moreover, we found MALII is required α2,6-sialylation for binding disialyl-T structures by the experiment with sialidase against a report26 which α2,6-sialylation in disialyl-T does not seem to be required for MALII binding to disialyl-T.
In this study, we focused on podoplanin, a disialyl-T glycosylated protein. It plays vital roles during developmental and postnatal stages. Podoplanin KO mice die after birth due to respiratory failure, with defective differentiation of type I alveolar cells10. Furthermore, it has been reported that Clec-2 KO mice also show abnormal lung development; additionally, the analysis of tissue-specific podoplanin KO mice (Tie2-Cre, podoplaninfl/fl) strongly suggests that the interaction of Clec-2 on platelets with podoplanin on lymphatic endothelial cells of the lung contributes to lung development33. Podoplanin KO mice also show abnormalities in lymphatic vessels due to the failure of blood–lymphatic vessel separation, resulting in blood-filled lymphatic vessels, edema, and blood–lymphatic discontinuities11,13,34,35,36. The blood–lymphatic vessel-mixed phenotype is also common in mice lacking C1galt1, a glycosyltransferase that regulates mucin-type O-glycosylation, or inactivated C1galt137,38,39. Both podoplanin- and Clec-2-deficient mice also show defects in neurovascular integrity in the embryonic brain40. In contrast to our expectations, no abnormalities were observed in the lung or lymphatic vessel development of DKO mice, although reduced reactivity was observed in the lungs of DKO mice using MALII staining. Bleeding abnormalities were observed in the lymph nodes after birth, indicating that DKO mice were healthy during the embryonic stage. These findings indicate that the disialyl-T structures modified by St6galnac3 and St6galnac4 do not regulate the lymphatic system during the embryonic stage but are important for the maintenance of vascular structures in HEV of the lymph nodes during the adult stage. Moreover, the single deletions of St6galnac3 and St6galnac4 did not prevent disialyl-T synthesis in various tissues, suggesting that the other enzyme synthesizes disialyl-T in a compensatory manner.
The function of podoplanin in homeostasis during the postnatal period has been reported based on the analysis of various podoplanin conditional KO mice. Blood-filled lymph nodes have also been observed in postnatal lymphatic endothelial cell-specific (Prox1-Cre-ERT2) podoplanin KO mice32. These hemorrhages were attributed to the retrograding of the subclavian vein into the thoracic duct due to the abnormality in the lymphovenous valves. In addition, after birth, the mice with Clec-2-deficient platelets do not show hemorrhage in the lymph nodes, although hemorrhage is observed in their lymph vessels41. Therefore, the absence of hemorrhage in the thoracic duct and mesenteric lymphatic vessels in DKO mice suggests that the hemorrhage in lymph nodes is independent of the hemorrhage in lymphatic vessels.
Another Clec-2-podoplanin-mediated mechanism in lymph nodes is the interaction between platelet Clec-2 and podoplanin in fibroblastic reticular cells, which contributes to the integrity of HEVs22. According to this report, the platelets activated by podoplanin-Clec-2 interactions induce the expression of Sphingosine-1-phosphate, followed by VE-cadherin in HEVs. Consistent with these findings, we observed a decrease in VE-cadherin within the HEV region in DKO mice. The decrease in VE-cadherin expression was more severe in podoplanin KO or Clec-2 KO than in DKO mice, as was the extent of the hemorrhages. The MALII lectin reactivity of the podoplanin protein purified by immunoprecipitation was lost only in DKO mice compared to that in WT and other single KO mice, suggesting that the disialyl-T structure of podoplanin was largely absent in DKO mice. However, podoplanin and Clec-2 protein levels in DKO mice were similar to those in WT mice. The differences in the extent of hemorrhage and the decrease in VE-cadherin expression may have been a result of alteration in disialyl-T structures. Since Clec-2 recognizes both the amino acids and glycosylation of the podoplanin protein7,8, we suspect that the loss of glycosylation may have partially inhibited these interactions in DKO mice, resulting in the impairment of lymph nodes (Fig. S7). The mechanism of lymph node hemorrhage is different from that of lymphatic hemorrhage41. Our results are consistent with this finding, as we observed only lymph node hemorrhage in DKO mice, without any signs of lymphatic hemorrhage.
While previous studies have elucidated the function of podoplanin, in the present study, we evaluated the role of glycans in the podoplanin-Clec-2 interaction in vivo by altering the glycan structure of podoplanin. The loss of the disialyl-T structure in podoplanin was observed not only in the lymph nodes but also in the kidney. Hence, podoplanin may function as a signaling molecule rather than just a ligand of Clec-2 in other tissues, even during embryonic development, owing to the abnormalities in these tissues during the embryonic stage, as seen in podoplanin KO mice and Clec-2 KO mice, which were not observed in DKO mice. However, as highlighted in this study, it is important to identify the glycosyltransferases that can synthesize glycan structures in individual organisms, such as mice, and determine the role of these glycans in the cell–cell communication. Disialyl-T, which is expressed in some cancer cells, is also involved in cancer cell invasion and migration6. Studies have also reported the role of podoplanin-Clec-2 interactions in cancer metastasis and prognosis23,24.
Furthermore, podoplanin derived from glioma cells exacerbates platelet aggregation in tumors in a mouse model42. Therefore, targets that inhibit the association between CLEC-2 and podoplanin are suitable candidates for the treatment of anticancer metastasis and anticancer-related thrombosis. However, since podoplanin is also expressed in normal tissues, it is necessary to be aware of the possibility of adverse drug effects. Glycans may be more effective targets for therapy than the proteins themselves, due to their various similar structures and the presence of tissue-specific complementary glycosyltransferases, which may compromise the function of the proteins themselves. In fact, useful some monoclonal antibodies, LpMab, has been reported to specifically recognize abnormally glycosylated podoplanin on cancer cells43. This study showed that α 2,6-sialylation of the disialyl-T structures is reduced in vivo by the loss of St6galnac3 and 4. This may be used to artificially induce changes in the disialyl-T structures by genetic modification. Furthermore, with regard to the disialyl-T structure of podoplanin, since only the abnormalities in lymph nodes were noticeable in vivo even after α 2,6-sialylation was reduced, it is possible that the side effects of losing this glycan function may be mild. Our results provide further evidence for targeting this interaction to develop therapeutic strategies to treat cancer.
The disialyl-T structure is a type of core 1-derived O-glycan; the incomplete elongation of core 1-derived O-glycans during development causes embryonic lethality due to the non-separation of blood and lymphatic vessels. The failure to prevent disialyl-T synthesis by the deletion of St6galnac3 and St6galnac4 is a limitation of this study, which can be addressed by analyzing phenotypic changes in St6galnac1,2,3,4 quadruple KO mice. We will ultimately investigate the substrate specificity and function of the St6galnac family in vivo, which is not yet clear. Moreover, we believe that these deficient mice would be useful for the evaluation of novel antibodies against cancer and for the assessment of metastasis and malignancy in vivo when cancer is induced.
Methods
Animals
Mice were maintained in specific pathogen-free conditions in the Laboratory Animal Resource Center at the University of Tsukuba. All experiments were performed in compliance with the relevant Japanese institutional laws and guidelines as well as approved by the University of Tsukuba Animal Ethics Committee (Authorization Number: 21-173). This experiment complied with the ARRIVE guidelines.
Generation of St6galnac3 and St6galnac4 knockout mice
The St6galnac3 and St6galnac4 KO mice were generated using the CRISPR/Cas9 system. In brief, we designed two target sgRNAs, 5′-GAATGTGTACTGCTCGTGCAGGG-3′ and 5′-CCCATCGATTCTGCCTTCTGTTC-3′, against regions located in introns 2 and 3, respectively, of the St6galnac3 gene of mouse chromosome 3, and two target sgRNAs, 5′-CCCCCAGGTGACCGACCCAGAGG-3′ and 5′-CCCGAATTCCATGTTCCGTTGC-3′, against region located in intron 1 and the untranslated region of exon 5, respectively, of the St6galnac4 gene of the mouse chromosome 2 (Fig. 1B). Each target sgRNA was cloned into the guide RNA-Cas co-expression plasmid px330-U6-Chimeric_BB-CBh-hSpCas9 vector (Addgene). A mixture of four cloned plasmids was microinjected into the pronuclei of C57BL/6 J fertilized eggs, and living embryos were transferred to pseudopregnant ICR female mice. Genomic DNA Screening was performed using PCR (Fig. 1C); the primers are listed in Table S3.
Antibodies and lectins
The details regarding antibodies and lectins used for immunofluorescence, flow cytometry, western blotting, immunoprecipitation, and dot blots are provided in Table S4.
Quantitative analysis of transcripts using RT-qPCR
The total RNA was extracted from various organs of C57BL/6 J and St6galnac-deficient mice using ISOGEN (Nippon Gene, Tokyo, Japan). First-strand cDNA was synthesized using the QuantiTect Reverse Transcription kit (Qiagen, Venlo, Netherland). PCR was performed using reagents from the THUNDERBIRD SYBR qPCR system (Toyobo, Osaka, Japan), and quantification was done using a TP850 Thermal Cycler Dice Real Time System (Takara Bio, Shiga, Japan). The primer sequences used for RT-qPCR are listed in Table S5. Zero blunt TOPO vectors (Thermo Fisher Scientific, Waltham, MA) cloned with DNA fragments of each PCR-amplified St6galnac gene were used as templates to create standard curves for absolute quantification of the St6galnac genes. The relative levels of transcripts of each St6galnac gene were normalized to those of the hypoxanthine guanine phosphoribosyl transferase (Hprt) transcripts.
Analysis of blood indices
Peripheral blood samples from 10- to 18-week-old male mice were obtained from the inferior vena cava and collected in polypropylene tubes containing 100 µl EDTA-2 K. Blood counts were determined using an automated hemocytometer (Nihon Kohden, Tokyo, Japan).
Flow cytometric analysis
The spleen and thymus were mechanically homogenized and passed through a 70 µm cell strainer. The cells were hemolyzed in ACK Lysing buffer (0.15 M NH4Cl, 10 mM KHCO3, 1 mM EDTA-2Na) at 23–26 °C for 5 min. Samples were washed three times with 1% Bovine serum albumin (BSA)/ Dulbecco’s phosphate buffered saline (DPBS; Thermo Fisher Scientific); 1 × 106 cells were incubated with each primary antibody against lymphocyte markers in 100 µL 1% BSA/DPBS for 20 min on ice. The cells were then washed twice with 1% BSA/DPBS. To remove dead cells, 4′,6-diamidino-2-phenylindole (DAPI, Dojindo Laboratories, Kumamoto, Japan) was added before flow cytometry. Flow cytometric analysis was performed using CytoFLEX (Beckman Coulter, Brea, CA).
Histological analysis
Tissues were fixed in Mildform 10 N (Fujifilm Wako, Osaka, Japan) for 16–20 h at 4 °C and then embedded in paraffin. Three micrometer-thick sections were stained with HE after deparaffinization. For immunohistochemical analysis, 3 µm-thick sections were blocked with 5% BSA/phosphate-buffered saline (PBS; 137 mM NaCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl) or a Carbo-free blocking solution (for lectin staining, Vector laboratories, Newark, CA) for 1 h at 23–26 °C after deparaffinization. Next, these sections were incubated with primary antibodies or lectins for 16–20 h at 4 °C. Samples were visualized using Alexa Flour fluorescence-conjugated secondary antibodies (Thermo Fisher Scientific). Nuclei were counterstained with Vibrance Antifade Mounting Medium containing DAPI (Vector laboratories). All sections were observed using a BIOREVO BZ-X800 microscope (Keyence, Osaka, Japan).
VE-cadherin fluorescence intensity was quantified using the hybrid cell count application (Keyence). After the HEV area with positive PNAd signals was manually encircled, the fluorescence intensity of VE-cadherin was automatically quantified in the PNAd-positive area.
Protein extraction and immunoprecipitation
Tissues were solubilized in lysis buffer (20 mM HEPES (pH 7.4), 1% Triton X-100) containing protease inhibitor cOmplete (Roche, Basel, Switzerland). The lysates were completely solubilized by pipetting or homogenizing using Biomasher II (Nippi, Tokyo, Japan), if necessary. Protein quantification was performed using the DC Protein Assay Kit (Bio-rad, Hercules, CA) and a microplate reader (Bio-Rad).
Total protein lysate (100 µg) was incubated with 10–20 µL Protein A/G PLUS-Agarose (Santa Cruz, Dallas, TX, sc-2003) at 4 °C for 2 h to reduce non-specific binding. Then, the samples were incubated with anti-podoplanin or control anti-rat immunoglobulin G antibodies for 16–20 h at 4 °C and immunoprecipitated using Protein A/G PLUS-Agarose at 4 °C for 1 h. The reaction solution was washed three times with lysis buffer containing a protein inhibitor. The solution was reduced using SDS sample buffer at 95 °C for 5 min.
Western blotting and lectin blotting
The tissue extracts were separated on 7.5% or 10% SDS–polyacrylamide gels using the ERICA XV PANTERA SYSTEM (DRC, Osaka, Japan) and then transferred to Immobilon-P membrane (MilliporeSigma, Burlington, MA) using a wet blotting system (Nihon Eido, Tokyo, Japan). After blocking with 5% skim milk/PBS containing 0.1% Tween 20 (PBS-T) or a Carbo-free blocking solution containing 0.1% Tween 20 for 1 h at 23–26 °C, the membrane was incubated with primary antibodies/1% skim milk/PBS-T or lectins/blocking buffer for 16–20 h at 4 °C. The membrane was washed three times with PBS-T for 5 min and further incubated with secondary antibodies for 1 h at 23–26 °C. After washing three times with PBS-T, immunoblots were developed using Immobilon western chemiluminescent horseradish peroxidase substrate (MilliporeSigma) and visualized in the iBright imaging system (Thermo Fisher Scientific).
Dot blot analysis
The brain extracts were incubated with α2-3,6,8,9 Neuraminidase A (New England labs, Ipswich, MA, P0722S) derived from Arthrobacter ureafaciens or reaction buffer for 1 h at 37 °C. The treated extracts were dotted onto the Amersham Hybond ECL (Cytiva, Tokyo, Japan, RPN82D). The membrane was dried for 30 min at 23–26 °C and then wetted with PBS-T. After blocking with a Carbo-free blocking solution containing 0.1% Tween 20 for 1 h at 23–26 °C, the membrane was incubated with each lectin/blocking buffer for 16–20 h at 4 °C. The membrane was washed three times with PBS-T for 5 min and then incubated with streptavidin-HRP/blocking buffer for 1 h at 23–26 °C. After washing three times with PBS-T for 5 min, immunoblots were developed using the Immobilon western chemiluminescent horseradish peroxidase substrate (MilliporeSigma) and visualized in the iBright imaging system (Thermo Fisher Scientific). The intensity of each dot was quantified by the plugin tool Band/Peak Quantification Tool44 of ImageJ (version 1.53a, Wayne Rasband, NIH, USA).
Statistical analysis
Statistical analyses were performed using Prism 7 (version 7.0e, GraphPad Software, San Diego, CA), with statistical significance set at 5% (p < 0.05). Data variation shown in the figures is represented as the standard error of the mean (SEM).
References
Christiansen, M. N. et al. Cell surface protein glycosylation in cancer. Proteomics 14, 525–546. https://doi.org/10.1002/pmic.201300387 (2014).
Pearce, O. M. & Laubli, H. Sialic acids in cancer biology and immunity. Glycobiology 26, 111–128. https://doi.org/10.1093/glycob/cwv097 (2016).
Varki, A. Biological roles of glycans. Glycobiology 27, 3–49. https://doi.org/10.1093/glycob/cww086 (2017).
Kudelka, M. R., Ju, T., Heimburg-Molinaro, J. & Cummings, R. D. Simple sugars to complex disease–mucin-type O-glycans in cancer. Adv. Cancer Res. 126, 53–135. https://doi.org/10.1016/bs.acr.2014.11.002 (2015).
Hugonnet, M., Singh, P., Haas, Q. & von Gunten, S. The distinct roles of sialyltransferases in cancer biology and onco-immunology. Front. Immunol. 12, 799861. https://doi.org/10.3389/fimmu.2021.799861 (2012).
Dimitroff, C. J. Galectin-binding O-glycosylations as regulators of malignancy. Cancer Res. 75, 3195–3202. https://doi.org/10.1158/0008-5472.CAN-15-0834 (2015).
Kato, Y. et al. Molecular analysis of the pathophysiological binding of the platelet aggregation-inducing factor podoplanin to the C-type lectin-like receptor CLEC-2. Cancer Sci. 99, 54–61. https://doi.org/10.1111/j.1349-7006.2007.00634.x (2008).
Nagae, M. et al. A platform of C-type lectin-like receptor CLEC-2 for binding O-glycosylated podoplanin and nonglycosylated rhodocytin. Structure 22, 1711–1721. https://doi.org/10.1016/j.str.2014.09.009 (2014).
Fuseya, S. et al. Mice lacking core 1-derived O-glycan in podocytes develop transient proteinuria, resulting in focal segmental glomerulosclerosis. Biochem. Biophys. Res. Commun. 523, 1007–1013. https://doi.org/10.1016/j.bbrc.2020.01.033 (2020).
Ramirez, M. I. et al. T1alpha, a lung type I cell differentiation gene, is required for normal lung cell proliferation and alveolus formation at birth. Dev. Biol. 256, 61–72. https://doi.org/10.1016/s0012-1606(02)00098-2 (2003).
Schacht, V. et al. T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema. EMBO J. 22, 3546–3556. https://doi.org/10.1093/emboj/cdg342 (2003).
Mahtab, E. A. et al. Cardiac malformations and myocardial abnormalities in podoplanin knockout mouse embryos: Correlation with abnormal epicardial development. Dev. Dyn. 237, 847–857. https://doi.org/10.1002/dvdy.21463 (2008).
Uhrin, P. et al. Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation. Blood 115, 3997–4005. https://doi.org/10.1182/blood-2009-04-216069 (2010).
Peters, A. et al. Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation. Immunity 35, 986–996. https://doi.org/10.1016/j.immuni.2011.10.015 (2011).
Krishnan, H. et al. Podoplanin: An emerging cancer biomarker and therapeutic target. Cancer Sci. 109, 1292–1299. https://doi.org/10.1111/cas.13580 (2018).
Quintanilla, M., Montero-Montero, L., Renart, J. & Martin-Villar, E. Podoplanin in inflammation and cancer. Int. J. Mol. Sci. 20, 707. https://doi.org/10.3390/ijms20030707 (2019).
Suzuki-Inoue, K. et al. Essential in vivo roles of the C-type lectin receptor CLEC-2: Embryonic/neonatal lethality of CLEC-2-deficient mice by blood/lymphatic misconnections and impaired thrombus formation of CLEC-2-deficient platelets. J. Biol. Chem. 285, 24494–24507. https://doi.org/10.1074/jbc.M110.130575 (2010).
Acton, S. E. et al. Podoplanin-rich stromal networks induce dendritic cell motility via activation of the C-type lectin receptor CLEC-2. Immunity 37, 276–289. https://doi.org/10.1016/j.immuni.2012.05.022 (2012).
Hess, P. R. et al. Platelets mediate lymphovenous hemostasis to maintain blood-lymphatic separation throughout life. J. Clin. Invest. 124, 273–284. https://doi.org/10.1172/JCI70422 (2014).
Benezech, C. et al. CLEC-2 is required for development and maintenance of lymph nodes. Blood 123, 3200–3207. https://doi.org/10.1182/blood-2013-03-489286 (2014).
Nakamura-Ishizu, A., Takubo, K., Kobayashi, H., Suzuki-Inoue, K. & Suda, T. CLEC-2 in megakaryocytes is critical for maintenance of hematopoietic stem cells in the bone marrow. J. Exp. Med. 212, 2133–2146. https://doi.org/10.1084/jem.20150057 (2015).
Herzog, B. H. et al. Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2. Nature 502, 105–109. https://doi.org/10.1038/nature12501 (2013).
Shirai, T. et al. C-type lectin-like receptor 2 promotes hematogenous tumor metastasis and prothrombotic state in tumor-bearing mice. J. Thromb. Haemost. 15, 513–525. https://doi.org/10.1111/jth.13604 (2017).
Suzuki-Inoue, K. Roles of the CLEC-2-podoplanin interaction in tumor progression. Platelets https://doi.org/10.1080/09537104.2018.1478401 (2018).
Kurosawa, N., Kojima, N., Inoue, M., Hamamoto, T. & Tsuji, S. Cloning and expression of Gal beta 1,3GalNAc-specific GalNAc alpha 2,6-sialyltransferase. J. Biol. Chem. 269, 19048–19053 (1994).
Geisler, C. & Jarvis, D. L. Effective glycoanalysis with Maackia amurensis lectins requires a clear understanding of their binding specificities. Glycobiology 21, 988–993. https://doi.org/10.1093/glycob/cwr080 (2011).
Ohmi, Y. et al. Majority of alpha2,6-sialylated glycans in the adult mouse brain exist in O-glycans: SALSA-MS analysis for knockout mice of alpha2,6-sialyltransferase genes. Glycobiology 31, 557–570. https://doi.org/10.1093/glycob/cwaa105 (2021).
Williams, S. E. et al. Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues. Nat. Commun. 13, 275. https://doi.org/10.1038/s41467-021-27781-9 (2022).
Kuno, A. et al. Focused differential glycan analysis with the platform antibody-assisted lectin profiling for glycan-related biomarker verification. Mol. Cell Proteomics 8, 99–108. https://doi.org/10.1074/mcp.M800308-MCP200 (2009).
Takeshita, M. et al. Alteration of matrix metalloproteinase-3 O-glycan structure as a biomarker for disease activity of rheumatoid arthritis. Arthritis Res. Ther. 18, 112. https://doi.org/10.1186/s13075-016-1013-2 (2016).
Osada, M. et al. Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells. J. Biol. Chem. 287, 22241–22252. https://doi.org/10.1074/jbc.M111.329987 (2012).
Bianchi, R. et al. Postnatal deletion of podoplanin in lymphatic endothelium results in blood filling of the lymphatic system and impairs dendritic cell migration to lymph nodes. Arterioscler Thromb. Vasc. Biol. 37, 108–117. https://doi.org/10.1161/ATVBAHA.116.308020 (2017).
Tsukiji, N. et al. Platelets play an essential role in murine lung development through Clec-2/podoplanin interaction. Blood 132, 1167–1179. https://doi.org/10.1182/blood-2017-12-823369 (2018).
Navarro-Nunez, L., Langan, S. A., Nash, G. B. & Watson, S. P. The physiological and pathophysiological roles of platelet CLEC-2. Thromb. Haemost. 109, 991–998. https://doi.org/10.1160/TH13-01-0060 (2013).
Pan, Y. & Xia, L. Emerging roles of podoplanin in vascular development and homeostasis. Front. Med. 9, 421–430. https://doi.org/10.1007/s11684-015-0424-9 (2015).
Suzuki-Inoue, K., Osada, M. & Ozaki, Y. Physiologic and pathophysiologic roles of interaction between C-type lectin-like receptor 2 and podoplanin: Partners from in utero to adulthood. J. Thromb. Haemost. 15, 219–229. https://doi.org/10.1111/jth.13590 (2017).
Xia, L. et al. Defective angiogenesis and fatal embryonic hemorrhage in mice lacking core 1-derived O-glycans. J. Cell Biol. 164, 451–459. https://doi.org/10.1083/jcb.200311112 (2004).
Fu, J. et al. Endothelial cell O-glycan deficiency causes blood/lymphatic misconnections and consequent fatty liver disease in mice. J. Clin. Invest. 118, 3725–3737. https://doi.org/10.1172/JCI36077 (2008).
Wang, Y. et al. Cosmc is an essential chaperone for correct protein O-glycosylation. Proc. Natl. Acad. Sci. U. S. A. 107, 9228–9233. https://doi.org/10.1073/pnas.0914004107 (2010).
Lowe, K. L. et al. Podoplanin and CLEC-2 drive cerebrovascular patterning and integrity during development. Blood 125, 3769–3777. https://doi.org/10.1182/blood-2014-09-603803 (2015).
Haining, E. J. et al. Lymphatic blood filling in CLEC-2-deficient mouse models. Platelets 32, 352–367. https://doi.org/10.1080/09537104.2020.1734784 (2021).
Costa, B. et al. Intratumoral platelet aggregate formation in a murine preclinical glioma model depends on podoplanin expression on tumor cells. Blood Adv. 3, 1092–1102. https://doi.org/10.1182/bloodadvances.2018015966 (2019).
Yamada, S. et al. LpMab-23: A cancer-specific monoclonal antibody against human podoplanin. Monoclon. Antib. Immunodiagn. Immunother. 36, 72–76. https://doi.org/10.1089/mab.2017.0001 (2017).
Ohgane, K. & Yoshioka, H. Quantification of gel bands by an Image J macro, band/peak quantification tool. Protocols.io (2019). https://doi.org/10.17504/protocols.io.7vghn3w
Acknowledgements
We would like to thank the staff at the Laboratory Animal Resource Center University of Tsukuba for their help in breeding and rearing the mice. We would like to thank Editage (www.editage.com) for English language editing. This work was supported by Grants-in-Aid for Scientific Research, JSPS KAKENHI (Grant Numbers 21K05984 and 21J11236).
Author information
Authors and Affiliations
Contributions
Conceptualization: S.F., S.T., and T.K.; formal analysis, S.F., H.I., and A.H.; data curation: S.F., H.I., and T.K.; investigation: S.F., H.I., A.H., Y.M., T.K., R.S., R.K., and T.H.; writing—original draft and figures preparation: S.F.; writing—review and editing: S.T. and T.K.; funding acquisition: S.F., S.T., and T.K.; supervision: S.T., and A.K. All authors have read and agreed to the published version of the manuscript.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Fuseya, S., Izumi, H., Hamano, A. et al. Reduction in disialyl-T antigen levels in mice deficient for both St6galnac3 and St6galnac4 results in blood filling of lymph nodes. Sci Rep 13, 10582 (2023). https://doi.org/10.1038/s41598-023-37363-y
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-023-37363-y
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.