Custom Using single-cell proteomics and transcriptomics to understand gene regulation

Date: This event took place on January 28, 2021

Custom webcast sponsor retains sole responsibility for content.

The fate and physiology of individual cells are controlled by proteins. Yet, the ability to quantitatively analyze proteins in single cells has remained limited. Seeking to overcome this barrier, Northeastern University’s Nikolai Slavov Lab and its single-cell proteomics center developed SCoPE2.

In this webcast, Dr. Slavov will describe how this method can increase quantitative accuracy and throughput while lowering cost and hands-on time by introducing automated and miniaturized sample preparation, enabling analysis of the emergence of cellular heterogeneity as homogeneous monocytes differentiated into macrophage-like cells in the absence of polarizing cytokine.

He will demonstrate how SCoPE2 quantified over 3,042 proteins in 1,490 single monocytes and macrophages in ten days of instrument time, allowing the team to discern single cells by cell type. Furthermore, the data uncovered a continuous gradient of proteome states for the macrophage-like cells, suggesting that macrophage heterogeneity may emerge even in the absence of polarizing cytokines.

Dr. Slavov will discuss how parallel measurements of transcripts by scRNA-seq suggest that SCoPE2 measurements can sample 20-fold more protein copies than RNA copies per gene, supporting quantification with improved count statistics, and how joint analysis of the data illustrates what variability across single cells can reveal about transcriptional and post-transcriptional gene regulation. The speaker will also reveal how this methodology lays a foundation for automated and quantitative single-cell analysis of proteins by mass spectrometry.

You will learn:

  • How to quantitatively analyse proteins in single cells
  • How to set up automation and miniaturize sample prep
  • How to jointly analyse transcriptomics and proteomics data from single cells

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This webcast has been produced for Thermo Fisher Scientific  by Nature Research Custom Media. The sponsor retains sole responsibility for content. About this content.

Speakers

  • Dr. Nikolai Slavov, Northeastern University

    Dr. Nikolai Slavov, Northeastern University

    Nikolai Slavov studied biology and physics at MIT before completing a dissertation at Princeton. Dr. Slavov returned to MIT for post-doctoral research focused on the coordination of metabolism and gene expression. His laboratory develops single-cell mass spectrometry methods, studies protein synthesis regulation by specialized ribosomes, and organizes the annual single-cell proteomics conference: single-cell.net.

  • Sarah Hiddleston, Science Journalist

    Moderator: Sarah Hiddleston, Science Journalist

    Sarah Hiddleston is freelance journalist working with Nature Research for Nature Middle East since 2015. Previously, Sarah worked for a decade in Madras (Chennai), India, specialising in health, pharmaceutical and environmental stories. Sarah holds an MA in Investigative Journalism from City University London, an MSc in Political Theory from the London School of Economics, and an undergraduate degree in History from the University of Cambridge, UK.