Custom Releasable purification of monocytes for human dendritic cell generation

Date: This event took place on April 27, 2016

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Dendritic cells (DCs) are an indispensable part of studying human responses that are important for protective immunity against cancer and infectious diseases as well as prevention of autoimmunity and transplant rejection.  These cells are also key elements of personalized vaccines which are a major research focus in cancer and infectious diseases.

Due to dendritic cells being present in very low concentrations in blood, less than 1%, these cells must be generated from monocytes.  This process is accomplished via a well understood technique in which cells are cultured in a differentiation medium containing IL4 and GM-CSF over a period of 7 days to obtain immature dendritic cells which can then be stimulated using protocols specific to each laboratory, assay, or application.

A common starting step in this process is to obtain peripheral blood mononuclear cells (PMBCs) from whole blood via Ficoll separation and then enrich for monocytes simply by plastic adherence.  While this approach is sufficient for many dendritic cell assays, more specialized assays, relevant for virology for example, require a highly pure starting population of monocytes in order to minimize the presence of contaminating PBMCs in downstream assays.

In this webcast Dr Shashi K. Murthy will discuss how MagCloudz can be utilized to purify monocytes from human PBMCs in a robust and reproducible manner and enable magnetic bead-free generation of dendritic cells.

In this webcast you will learn:
• How MagCloudz can be used for CD14+ monocyte purification from blood.
• Key workflow and downstream benefits of MagCloudz Streptavidin Cell Separation Kit.
• How MagCloudz is differentiated from other cell separation technologies

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Speakers

  • Dr Shashi K. Murthy, Northeastern University

    Dr Shashi K. Murthy, Northeastern University

    Dr Murthy’s laboratory specializes in the design of instrumentation and reagents to address needs in immunology, stem cell biology, and medical devices. He is a Professor of Chemical Engineering and the Founding Director of the Sherman Center for Engineering Entrepreneurship Education at Northeastern University. In addition, Dr Murthy is a co-founder and Chief Scientific Advisor of Quad Technologies. Dr Murthy earned his Ph.D. at MIT, his B.S. at the Johns Hopkins University and postdoctoral training at the Harvard Medical School and Massachusetts General Hospital. He is the recipient of the U.S. National Science Foundation’s Faculty Early Career Development (CAREER) Award and the Søren Buus Award for Outstanding Research in Engineering at Northeastern University. He was recognized as a Fellow of the American Institute for Medical and Biological Engineering (AIMBE) in 2016. Dr Murthy has co-authored over 70 publications and is an inventor on 7 issued or pending patents.

  • Moderator: Neil Bowdler

    Neil Bowdler is a freelance science reporter. He has worked for BBC TV, Radio and Online. His work includes the BBC special ‘How to put a human on Mars’ which won a WAN-IFRA World Digital Media Award. Previously he worked as the Warsaw correspondent of The Guardian. He studied at Cambridge University.