Date: This event took place on September 27, 2016
Förster resonance energy transfer (FRET) can be used to detect and quantify interactions between two molecules, or two parts of a molecule. Fluorescence microscopy is commonly used to detect these interactions. FRET has been applied to a variety of cell types and can thus be used to address a diverse range of biological questions. In this webcast we briefly over-view the theoretical principles of FRET and discuss a range of applications in which it has been used. Experts with extensive experience using FRET discuss the practical considerations that need to be considered when embarking on a study using FRET, for example, selection of fluorophore pairs and minimisation of imaging-induced artefacts. In addition, as inspiration for your own future research, hear about some occasions in which the researchers have used FRET in particularly innovative ways.
Please join us to learn more about how to practically implement FRET techniques and question our experts during our live Q and A session.
Prof. Ammasi Periasamy, University of Virginia
Dr. Periasamy is an internationally recognized expert in advanced microscopy techniques in bioimaging. He is one of the pioneers in the development of fluorescence lifetime imaging microscopy (FLIM) for measuring the oscillations in cytosolic and nuclear free calcium in single intact living cells. He developed a 2- and 3-color steady state, confocal, multiphoton, and FLIM based Förster resonance energy transfer (FRET) imaging system for protein localization in living specimens.
He runs an annual workshop on FLIM & FRET Microscopy.
Dr Margarida Barroso, Albany Medical College
Dr. Barroso is a faculty instructor on several international imaging courses. Dr. Barroso’s research group uses receptor-targeted approaches to visualize, quantitate and optimize drug delivery into cancer cells. By integrating knowledge of the endocytic trafficking of receptor-ligand complexes with expertise in Forster Resonance Energy Transfer (FRET) and fluorescence lifetime (FLIM) imaging, Dr. Barroso’s research group has developed novel in vivo and in vitro methods to measure receptor target engagement in tumors
Dr Klaus Hahn, University of North Carolina
Dr. Hahn’s laboratory develops new means to visualize and control protein activity in vivo, and uses them to study the role of precise signaling dynamics in immune cell decision making. His laboratory has produced biosensors that report the conformational changes of endogenous proteins, biosensors based on artificial protein scaffolds for difficult targets, and fluorescent dyes for microscopy of conformational changes in living cells. Most recently, his laboratory has focused on altering proteins to confer response to light or small molecules in vivo.
Dr Klaus Suhling, King’s College London
Klaus Suhling develops and uses advanced multidimensional fluorescence imaging techniques such as Fluorescence Lifetime Imaging (FLIM) to understand the properties and interactions of macromolecules in the life sciences. Additional techniques he uses include time-resolved fluorescence anisotropy imaging (tr-FAIM), fluorescence spectroscopy, time-correlated single photon counting (TCSPC) and photon counting imaging.