Custom Overcoming current challenges with 3D cell culturing

Date: This event took place on June 15, 2016

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3D culture is transforming cell biology and tissue engineering applications. However such evolution in research is also coupled with new challenges. 3D culture methods still require solid expertise in handling and optimisation to build good in vitro models, especially multilayer complex models. In addition, utilising 3D cultures with standard cell analysis methods, which were originally developed to support 2D cultures, can be challenging.

In this webcast, we explain approaches of how to overcome these challenges. The RAFT™ 3D Culture System has been used to develop complex multilayer cell culture models with different cell types. In addition, protocols are available that explain how to apply standard 2D cell analysis techniques easily in RAFT 3D Cultures.

Dr. Julie T. Daniels will explain how her lab at the University College London has developed multilayer corneal models using these cultures and is using this 3D method to address the current gaps in limbal stem cell deficiency (LSCD) treatment. Her lab aspires to take their research to the next level into clinical applications.

Dr. Jenny Schroeder will summarise other recent advances in 3D cell culture – in particular the development of novel blood-brain-barrier and liver fibrosis models. She will also elaborate on how to apply standard cell analysis techniques such as fluorescence microscopic imaging and viability assays on RAFT 3D Cultures.

In this webcast you will learn:

  • How to apply cell analysis tools to 3D cultures.
  • How 3D methods are elevating research into translational applications.
  • How to develop multilayer 3D models using a novel 3D method.


  • Dr. Julie T. Daniels, University College London, UK

    Dr. Julie T. Daniels, University College London, UK

    Julie T. Daniels is the founder and director of the Cells for Sight Stem Cell Therapy Research Unit of the UCL Institute of Ophthalmology. She is a leader in translational research on the use of stem cells for repair of the human ocular surface. The goal of Julie’s team is to tissue engineer components of human cornea for therapeutic transplantation, experimental investigation and drug discovery.

  • Dr. Jenny Schroeder, Lonza Cologne GmbH, Germany

    Dr. Jenny Schroeder, Lonza Cologne GmbH, Germany

    As a Senior Scientist Dr. Jenny Schroeder is leading Lonza research teams based in Cologne, Germany and Hyderabad, India that are developing innovative research tools for applied bioresearch and drug discovery. More physiological 3D cell culture systems and advanced transfection methods are main research interests.

  • Dr. Jayshan Carpen, Springer Nature

    Moderator: Dr. Jayshan Carpen, Springer Nature

    Jayshan is a Senior Publishing Manager for Springer Nature and oversees the custom multimedia unit. Previously he ran science events at the Royal Institution of Great Britain. He received his PhD from the University of Surrey, UK in Neurogenetics. His doctoral thesis focused on identifying polymorphisms associated with diurnal preference and circadian sleep disorders.