Date: This event took place on March 23, 2017
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In situ hybridization (ISH) has become a mainstay of many approaches to understanding gene expression in biology and pathology, whereby labelled probes complementary to mRNA sequences – i.e., anti-sense probes – are used to localize mRNAs in cells and tissue sections. In existence for some 5 decades, the basic ISH technique still requires substantial optimization covering a range of skills, from basic molecular biology to tissue isolation and preparation, and is subject to further variability amongst operators. RNAscope offers a superior alternative and scalable approach to classical ISH, with recent iterations allowing resolution of much shorter targets and even junctional sequences.
This webcast provides an introduction to this comparatively new commercial technology and its use in answering questions previously addressed by classical techniques. It is presented from the viewpoint of an ISH user with 30-years of classical experience who has switched to RNAscope and BaseScope, with a particular emphasis on its use in studying the nervous system. As a tangible example, the use of RNAscope was instrumental in validating “hits” identified from interrogating single-neuron transcriptomes obtained by sequencing single neuronal nuclei of the human brain (Science 352, 1586-1590 (2016)), and examples from this and other research efforts will be presented.
In this webcast you will learn;
- Advances in situ hybridization and RNAscope enable RNA gene expression analysis off-the-shelf and single day assays
- BaseScope gene expression analysis enables splice variance and short target analysis
- mRNA analysis in neuroscience research where no antibodies are available
- Detection of long non-coding RNA (lncRNA)
- RNA-seq to target validation and validation of (cell type-specific) genetic modifications
- Ability to visualize in situ expression of virtually any gene, any species and any tissue
Dr. Jerold Chun, Sanford Burnham Prebys Medical Discovery Institute
Dr. Jerold Chun is Professor and Senior Vice President of Neuroscience Drug Discovery at Sanford Burnham Prebys Medical Discovery Institute. He received his MD/PhD (Neuroscience) degrees through the MSTP at Stanford University, pursued Postdoctoral training at the Whitehead Institute/MIT and has held professorships at UCSD, TSRI, and was Department head of Molecular Neuroscience at Merck, before joining SBP. He has used ISH techniques for close to 30 years.
Moderator: Dr. Jayshan Carpen, Springer Nature
Jayshan is a Senior Publishing Manager for Springer Nature and oversees the custom multimedia unit. Previously he ran science events at the Royal Institution of Great Britain. He received his PhD from the University of Surrey, UK in Neurogenetics. His doctoral thesis focused on identifying polymorphisms associated with diurnal preference and circadian sleep disorders.