Wide-field fluorescence microscopy articles from across Nature Portfolio

Random-access wide-field mesoscopy enables the imaging of in vivo biodynamics in mice over an area of 160 mm² and at a subcellular spatial resolution of about 2 μm.

A pretrained foundation model (UniFMIR) enables versatile and generalizable performance across diverse fluorescence microscopy image reconstruction tasks.

The authors present an adaptable ex vivo animal model to study deep tissue injury development by providing accurate quantitative data on mechanically induced cell damage in skeletal muscle tissue.

Current systems for imaging calcium dynamics in the brains of non-human primates require the animal’s movement to be restricted. Here, the authors demonstrate a mesoscale calcium imaging device in a freely moving non-human primate which features a 20 mm² field of view.

To capture expansive, seamless fields of view from frozen hydrated specimens by cryo-electron tomography, we developed methods for the collection and processing of montage data. This approach enables rapid acquisition of contiguous regions of specimens using a montaged tilt series collection scheme.

The LED-integrated excitation cube (LEC) is compact, low-cost, and easy to install, taking full advantage of the benefits of LED excitations, and can be used in any fluorescent microscope.

The jellyfish-derived green fluorescent protein StayGold is bright and hardly fades, contributing to improving spatiotemporal resolution and dramatically extending the observation period. To fully benefit from the rich photon budget, we tried some unusual illumination modalities for sustainable, quantitative live-cell or volumetric imaging.

Advances in adaptive optics combined with a twist on light field microscopy enable high-quality imaging.

A wide-field system that can perform video-rate imaging of the entire area of the brain of an awake mouse is aiding the study of neurones, epilepsy and the immune system.